Laboratory for Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100 Xanthi, Greece.
Bioprocess Biosyst Eng. 2013 Jul;36(7):965-74. doi: 10.1007/s00449-012-0832-z. Epub 2012 Oct 2.
Biohydrogen production from a simulated fruit wastewater (soluble COD = 3.17 ± 0.10 g L⁻¹) was carried out in a continuous stirred tank reactor (CSTR) of 2 L operational volume without biomass inoculation, heat pre-treatment or pH adjustment, resulting in a low operational pH (3.75 ± 0.09). The hydraulic retention time (HRT) varied from 15 to 5 h. A strong negative correlation (p < 0.01) between the biogas production rate and the HRT was observed. Biogas production rates were higher at 30 °C than at 25 °C (p < 0.01), when the CSTR was operated under the same HRT. The biogas hydrogen content was estimated as high as 55.8 ± 2.3 % and 55.4 ± 2.5 % at 25 and 30 °C, respectively. The main fermentation end products were acetic and butyric acids, followed by ethanol. Significant differences (p < 0.01) during the operation of the CSTR at 25 or 30 °C were identified for butyric acid at almost all HRTs examined. Simulation of the acidogenesis process in the CSTR (based on COD and carbon balances) indicated the possible metabolic compounds produced at 25 and 30 °C reactions and provided an adequate fit of the experimental data.
采用 2 L 有效容积的连续搅拌槽式反应器（CSTR），在不接种生物质、不进行热预处理或不调节 pH 值的情况下，从模拟水果废水中（可溶解 COD = 3.17 ± 0.10 g L⁻¹）生产生物氢气，导致操作 pH 值较低（3.75 ± 0.09）。水力停留时间（HRT）从 15 小时变化到 5 小时。观察到沼气产生率与 HRT 之间存在强烈的负相关关系（p < 0.01）。当 CSTR 在相同 HRT 下运行时，沼气产生率在 30°C 时高于 25°C（p < 0.01）。沼气中氢气的含量估计高达 55.8 ± 2.3%和 55.4 ± 2.5%，分别在 25°C 和 30°C 时。主要发酵终产物为乙酸和丁酸，其次为乙醇。在 25°C 或 30°C 下运行 CSTR 时，几乎在所有检查的 HRT 下，丁酸都存在显著差异（p < 0.01）。基于 COD 和碳平衡，对 CSTR 中的产酸过程进行模拟表明，在 25°C 和 30°C 反应中可能产生了代谢化合物，并对实验数据进行了充分拟合。
