Levine R A, Schwartzel E H, Randall P A, Bachman S
Prostaglandins. 1979 Jul;18(1):63-72. doi: 10.1016/s0090-6980(79)80024-6.
The action of prostaglandins and indomethacin on gastric mucosal cyclic nucleotide concentrations was evaluated in 18 anesthetized mongrel dogs. Prostaglandins E1 (PGE1) and E2 (PGE2) (25 microgram/kg bolus, then 2 micrograms/kg/min) were administered both intravenously (4 experiments; femoral vein) and directly into the gastric mucosal circulation (10 experiments; superior mesenteric artery). The possible synergistic effect of pre-treatment and continuous arterial infusion of indomethacin (5 mg/kg bolus for 5 min, then 5 mg/min), a prostaglandin synthetase inhibitor, with PGE2 was studied in 4 experiments. Antral and fundic mucosa were biopsied and measured by radioimmunoassay for cyclic nucleotides. Doses of PGE1 and PGE2 which inhibited histamine-stimulated canine gastric acid secretion did not significantly alter antral or fundic mucosal cyclic nucleotide concentrations. Concomitant infusion of PGE2 with indomethacin did not potentiate the mucosal nucleotide response compared to PGE2 alone. These studies fail to implicate cyclic nucleotides as mediators of the inhibitory acid response response induced by PGE1 or PGE2 in intact dog stomach.
在18只麻醉的杂种犬中评估了前列腺素和吲哚美辛对胃黏膜环核苷酸浓度的作用。前列腺素E1（PGE1）和E2（PGE2）（静脉注射25微克/千克推注剂量，然后以2微克/千克/分钟持续输注），静脉注射给药4次实验（股静脉），直接注入胃黏膜循环给药10次实验（肠系膜上动脉）。在4次实验中研究了预先给予前列腺素合成酶抑制剂吲哚美辛（5毫克/千克推注5分钟，然后5毫克/分钟）并持续动脉输注，与PGE2联合使用时可能产生的协同作用。取胃窦和胃底黏膜活检组织，采用放射免疫分析法测定环核苷酸。抑制组胺刺激犬胃酸分泌的PGE1和PGE2剂量，并未显著改变胃窦或胃底黏膜环核苷酸浓度。与单独使用PGE2相比，PGE2与吲哚美辛联合输注并未增强黏膜核苷酸反应。这些研究未能证明环核苷酸是完整犬胃中PGE1或PGE2诱导的胃酸抑制反应的介质。
