Kaminski D L, Ruwart M J, Deshpande Y G
Prostaglandins. 1979 Jul;18(1):73-82. doi: 10.1016/s0090-6980(79)80025-8.
The synthetic prostaglandin analogs 16, 16-dimethyl PGF2 alpha and 16, 16-dimethyl PGE2 were administered to dogs with chronic biliary and gastric fistulas. The effects of 16, 16 diMePGF2 alpha and 16, 16 diMePGE2 were evaluated on bile flow and composition and bile adenosine 3', 5' monophosphate (cyclic AMP) secretion. 16, 16 diMePGF2 alpha in doses of 0.125 and 0.25 microgram-kg-min significantly increased hepatic bile flow. The choleresis was characterized by increased chloride and bicarbonate secretion. Measurement by radioimmunoassay of bile cyclic AMP concentration demonstrated no evident increase in bile cyclic AMP secretion associated with the choleresis produced by 16, 16 diMePGF2 alpha. The administration of 16, 16 diMePGE2 in a dose range 0.01 to 1.0 microgram-kg-min did not significantly alter bile flow rates or composition. Bile erythritol-14C clearance, a measure of canalicular bile flow, was significantly increased by PGF2 alpha but not by 16, 16-dimethyl PGF2 alpha, suggesting that the mechanism of action of PGF2 alpha in stimulating hepatic bile flow may be different from that involved in 16, 16-dimethyl PGF2 alpha choleresis. The results of this study indicate that the synthetic PGF2 alpha analog produces a choleretic response not mediated by adenylate cyclase and associated with increased chloride and bicarbonate secretion.
将合成的前列腺素类似物16,16 - 二甲基前列腺素F2α和16,16 - 二甲基前列腺素E2给予患有慢性胆管和胃瘘的犬。评估16,16 - 二甲基前列腺素F2α和16,16 - 二甲基前列腺素E2对胆汁流量、成分及胆汁中3',5' - 单磷酸腺苷（环磷酸腺苷）分泌的影响。剂量为0.125和0.25微克/千克/分钟的16,16 - 二甲基前列腺素F2α显著增加肝胆汁流量。胆汁分泌增加的特征是氯化物和碳酸氢盐分泌增多。通过放射免疫测定法测量胆汁中环磷酸腺苷浓度表明，与16,16 - 二甲基前列腺素F2α引起的胆汁分泌增加相关的胆汁中环磷酸腺苷分泌无明显增加。剂量范围为0.01至1.0微克/千克/分钟的16,16 - 二甲基前列腺素E2给药并未显著改变胆汁流速或成分。前列腺素F2α可显著增加作为胆小管胆汁流量指标的胆汁赤藓糖醇 - 14C清除率，但16,16 - 二甲基前列腺素F2α则无此作用，这表明前列腺素F2α刺激肝胆汁流量的作用机制可能与16,16 - 二甲基前列腺素F2α引起胆汁分泌增加的机制不同。本研究结果表明，合成的前列腺素F2α类似物产生的胆汁分泌增加反应并非由腺苷酸环化酶介导，而是与氯化物和碳酸氢盐分泌增加有关。
