Błachnio-Zabielska Agnieszka U, Baranowski Marcin, Hirnle Tomasz, Zabielski Piotr, Lewczuk Anna, Dmitruk Iwona, Górski Jan
Department of Physiology, Medical University of Bialystok, Mickiewicza 2c, 15-222 Bialystok, Poland,
Lipids. 2012 Dec;47(12):1131-41. doi: 10.1007/s11745-012-3722-x. Epub 2012 Oct 10.
Obesity is a risk factor for metabolic diseases. Intramuscular lipid accumulation of ceramides, diacylglycerols, and long chain acyl-CoA is responsible for the induction of insulin resistance. These lipids are probably implicated in obesity-associated insulin resistance not only in skeletal muscle but also in fat tissue. Only few data are available about ceramide content in human subcutaneous adipose tissue. However, there are no data on DAG and LCACoA content in adipose tissue. The aim of our study was to measure the lipids content in human SAT and epicardial adipose tissue we sought to determine the bioactive lipids content by LC/MS/MS in fat tissue from lean non-diabetic, obese non-diabetic, and obese diabetic subjects and test whether the lipids correlate with HOMA-IR. We found, that total content of measured lipids was markedly higher in OND and OD subjects in both types of fat tissue (for all p < 0.001) as compared to LND group. In SAT we found positive correlation between HOMA-IR and C16:0-Cer (r = 0.79, p < 0.001) and between HOMA-IR and C16:0/18:2 DAG (r = 0.56, p < 0.001). In EAT we found a strong correlation between C16:0-CoA content and HOMA-IR (r = 0.73, p < 0.001). The study showed that in obese and obese diabetic patients, bioactive lipids content is greater in subcutaneous and epicardial fat tissue and the particular lipids content positively correlates with HOMA-IR.
肥胖是代谢性疾病的一个风险因素。神经酰胺、二酰甘油和长链酰基辅酶A在肌肉内的脂质积累是诱导胰岛素抵抗的原因。这些脂质可能不仅在骨骼肌中，而且在脂肪组织中都与肥胖相关的胰岛素抵抗有关。关于人体皮下脂肪组织中神经酰胺含量的数据很少。然而，目前尚无关于脂肪组织中二酰甘油和长链酰基辅酶A含量的数据。我们研究的目的是测量人体皮下脂肪组织（SAT）和心外膜脂肪组织中的脂质含量，我们试图通过液相色谱/串联质谱法（LC/MS/MS）测定瘦的非糖尿病、肥胖的非糖尿病和肥胖的糖尿病受试者脂肪组织中的生物活性脂质含量，并测试这些脂质是否与胰岛素抵抗指数（HOMA-IR）相关。我们发现，与瘦的非糖尿病（LND）组相比，在两种类型的脂肪组织中，肥胖的非糖尿病（OND）和肥胖的糖尿病（OD）受试者中所测脂质的总含量均显著更高（所有p<0.001）。在SAT中，我们发现HOMA-IR与C16:0-神经酰胺（r = 0.79，p<0.001）以及HOMA-IR与C16:0/18:2二酰甘油（r = 0.56，p<0.001）之间呈正相关。在心外膜脂肪组织（EAT）中，我们发现C16:0-辅酶A含量与HOMA-IR之间存在强相关性（r = 0.73，p<0.001）。该研究表明，在肥胖和肥胖的糖尿病患者中，皮下和心外膜脂肪组织中的生物活性脂质含量更高，且特定脂质含量与HOMA-IR呈正相关。
