Department of Bioproducts and Biosystems Engineering, University of Minnesota, 316 BAE, 1390 Eckles Ave, Saint Paul, MN 55108-6005, USA.
Appl Biochem Biotechnol. 2012 Dec;168(7):1896-906. doi: 10.1007/s12010-012-9905-3. Epub 2012 Oct 6.
Hydrolytic enzymes were evaluated on the lipid accumulation via an oleaginous fungal species, Mortierella isabellina, cultivated on sugars released from soybean hulls. The weight loss of soybean hull, fungal growth, and lipid production were tested under different loads of hydrolytic enzymes. M. isabellina could not directly utilize cellulose and adding cellulase and β-glucosidase significantly increased the cell growth and oil accumulation of M. isabellina on soybean hulls. The highest weight loss of soybean hulls was 47.80 % and the lipid production reached 0.14 g from 1 g of soybean hull when 12 U cellulase, 27.2 U β-glucosidase, 2,278.56 U pectinase, and 15 U hemicellulase were added. Fatty acids (76.82 %) accumulated in M. isabellina were C16 and C18, which are suitable for biodiesel production. These results provide a new application for soybean hulls to be applied as the raw material for the production of biodiesel fuel, besides its traditional role as animal feed supplements.
水解酶被评估为通过一种产油真菌，即玫红弯孢霉，在从大豆皮中释放的糖上积累脂质。在不同水解酶负荷下测试了大豆皮的失重、真菌生长和油脂生产。玫红弯孢霉不能直接利用纤维素，添加纤维素酶和β-葡萄糖苷酶显著增加了玫红弯孢霉在大豆皮上的细胞生长和油脂积累。当添加 12 U 纤维素酶、27.2 U β-葡萄糖苷酶、2,278.56 U 果胶酶和 15 U 半纤维素酶时，大豆皮的失重率最高可达 47.80%，从 1 g 大豆皮中生产的油脂达到 0.14 g。在玫红弯孢霉中积累的脂肪酸（76.82%）为 C16 和 C18，适合生产生物柴油。这些结果为大豆皮的新应用提供了依据，除了作为动物饲料补充剂的传统作用外，大豆皮还可以用作生物柴油燃料生产的原料。
