Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory, Washington State University, LJ Smith 213, Pullman, WA 99163, United States.
Bioresour Technol. 2013 Jan;128:385-91. doi: 10.1016/j.biortech.2012.10.079. Epub 2012 Oct 26.
Various carbon sources including monosugars, disaccharides and carboxymethyl-cellulose (CMC) were used for single-cell oil production by the filamentous fungus Mortierella isabellina. In addition, the inhibitory effects of lignocellulose-derived compounds (lignin aldehydes, furan aldehydes and weak acid) were investigated. C6 sugars were preferably used for growth compared to C5 sugars. CMC was not an usable substrate, implying the absence of a cellulase system in this fungus. Lignin derivatives showed the most inhibitory effects, but acetic and formic acids at concentrations of 4 g/L improved lipid production, achieving 6.81 ± 0.07 g/L and 6.66 ± 0.33 g/L respectively, which was twice as high as that of the control. A 16.8% lipid yield from hydrolysate suggested that this fungus could be useful for microbial lipid production.
各种碳源，包括单糖、二糖和羧甲基纤维素（CMC），被用于丝状真菌美极毛霉的单细胞油脂生产。此外，还研究了木质纤维素衍生化合物（木质素醛、糠醛和弱酸）的抑制作用。与 C5 糖相比，C6 糖更有利于生长。CMC 不是一种可用的底物，这表明该真菌中不存在纤维素酶系统。木质素衍生物表现出最显著的抑制作用，但在 4 g/L 浓度下的乙酸和甲酸可提高油脂产量，分别达到 6.81±0.07 g/L 和 6.66±0.33 g/L，是对照的两倍。水解产物的 16.8%的油脂产率表明，该真菌可用于微生物油脂生产。
