Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Bioresour Technol. 2014 Jul;163:12-7. doi: 10.1016/j.biortech.2014.03.095. Epub 2014 Mar 27.
A combined hydrolysis process, which first mixed dilute acid- and alkali-pretreated corn stover at a 1:1 (w/w) ratio, directly followed by enzymatic saccharification without pH adjustment, has been developed in this study in order to minimize the need of neutralization, detoxification, and washing during the process of lignocellulosic biofuel production. The oleaginous fungus Mortierella isabellina was selected and applied to the combined hydrolysate as well as a synthetic medium to compare fungal lipid accumulation and biodiesel production in both shake flask and 7.5L fermentor. Fungal cultivation on combined hydrolysate exhibited comparable cell mass and lipid yield with those from synthetic medium, indicating that the integration of combined hydrolysis with oleaginous fungal lipid fermentation has great potential to improve performance of advanced lignocellulosic biofuel production.
本研究开发了一种组合水解工艺，首先将稀酸预处理和碱预处理的玉米秸秆以 1:1（w/w）的比例混合，直接进行酶解，无需 pH 调整，以尽量减少在木质纤维素生物燃料生产过程中中和、解毒和洗涤的需要。选择产油真菌枝顶孢霉（Mortierella isabellina）并将其应用于组合水解产物以及合成培养基中，以比较真菌油脂积累和生物柴油在摇瓶和 7.5L 发酵罐中的生产情况。在组合水解产物上进行真菌培养，其细胞质量和油脂产量与合成培养基相当，表明将组合水解与产油真菌油脂发酵相结合具有提高先进木质纤维素生物燃料生产性能的巨大潜力。
