Department of Forest Products Technology, School of Chemical Technology, Aalto University, 00076 Espoo, Finland.
Biointerphases. 2012 Dec;7(1-4):61. doi: 10.1007/s13758-012-0061-7. Epub 2012 Oct 3.
We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.
我们介绍了一种新的方法来修饰纳米原纤纤维素（NFC）薄膜，以生产用于诊断的无孔、防水基底。首先，通过机械分散 NFC 水凝胶制备了防水 NFC 薄膜，然后通过 TEMPO 介导的氧化使它们的表面羧化。接下来，通过 EDS/NHS 化学对拓扑功能化薄膜进行活化，并使用牛血清白蛋白和抗人 IgG 验证其反应性。使用石英晶体微天平（QCM-D）、接触角测量、电导滴定、X 射线光电子能谱（XPS）和荧光显微镜证实了表面羧化、EDC/NHS 活化和蛋白质附着。使用共聚焦激光扫描显微镜（CLSM）和原子力显微镜（AFM）研究了制备的薄膜的表面形态。最后，我们证明了可以使用商业压电喷墨打印将抗人 IgG 固定在活化的 NFC 表面上。
