Swiss Center for Electronics and Microtechnology (CSEM, Landquart), Bahnhofstrasse 1, 7302 Landquart, Switzerland.
Organs-on-Chip Technologies, ARTORG Center for Biomedical Engineering Research, University of Bern, Murtenstrasse 50, 3008 Bern, Switzerland.
Biosensors (Basel). 2020 Sep 13;10(9):125. doi: 10.3390/bios10090125.
Glucose sensors are potentially useful tools for monitoring the glucose concentration in cell culture medium. Here, we present a new, low-cost, and reproducible sensor based on a cellulose-based material, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidized-cellulose nanocrystals (CNCs). This novel biocompatible and inert nanomaterial is employed as a polymeric matrix to immobilize and stabilize glucose oxidase in the fabrication of a reproducible, operationally stable, highly selective, cost-effective, screen-printed glucose sensor. The sensors have a linear range of 0.1-2 mM (R = 0.999) and a sensitivity of 5.7 ± 0.3 µA cm∙mM. The limit of detection is 0.004 mM, and the limit of quantification is 0.015 mM. The sensor maintains 92.3 % of the initial current response after 30 consecutive measurements in a 1 mM standard glucose solution, and has a shelf life of 1 month while maintaining high selectivity. We demonstrate the practical application of the sensor by monitoring the glucose consumption of a fibroblast cell culture over the course of several days.
葡萄糖传感器在监测细胞培养液中的葡萄糖浓度方面具有潜在的应用价值。在此，我们提出了一种新的、低成本且可重现的传感器，它基于纤维素基材料 2,2,6,6-四甲基哌啶-1-氧基（TEMPO）氧化纤维素纳米晶体（CNC）。这种新型的生物相容性和惰性纳米材料被用作聚合物基质，以固定化和稳定葡萄糖氧化酶，从而制备出具有可重现性、操作稳定性、高选择性、经济高效且可丝网印刷的葡萄糖传感器。传感器的线性范围为 0.1-2 mM（R = 0.999），灵敏度为 5.7 ± 0.3 µA cm∙mM。检测限为 0.004 mM，定量限为 0.015 mM。传感器在 1 mM 标准葡萄糖溶液中连续测量 30 次后，仍保持初始电流响应的 92.3%，且具有 1 个月的保质期，同时保持高选择性。我们通过监测几天内成纤维细胞培养物的葡萄糖消耗，展示了传感器的实际应用。
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