Rashidi Mohammadreza, Aboomardani Maryam, Rafraf Maryam, Arefhosseini Seyed-Rafie, Keshtkar Abbasali, Joshaghani Hamidreza
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Pediatr. 2011 Mar;21(1):8-14.
In beta thalassemic patients, tissue damage occurs due to oxidative stress and it happens because of the accumulation of iron in the body. This study was conducted to determine the effect of zinc and vitamin E supplementation on antioxidant status in beta-thalassemic major patients.
This double blind randomized clinical trial was carried out on 120 beta thalassemic patients older than 18 years. Patients were randomly categorized in four groups. Zinc (50mg/day) and vitamin E (400mg/day) supplements were administered for former and latter group, respectively. In the third group both supplements were administered in similar doses. The fourth (control) group received no supplement. The effect of supplementations on serum zinc and vitamin E, superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC) and body mass index (BMI) were measured at the beginning and the end of the study.
Serum zinc levels in group 1 and 3 were significantly increased (P<0.007 and P<0.005, respectively). Serum vitamin E levels in group 2 and 3 were also increased significantly (P<0.001). Mean GPX activity in group1, 2 and 3 decreased significantly (P<0.015, P<0.032 and P<0.029, respectively). Mean SOD activity and TAC did not show significant change after supplementation. BMI had significant increase in all treated groups (P<0.001).
Our results suggest that beta thalassemic patients have enhanced oxidative stress and administration of selective antioxidants may preclude oxidative damage.
在β地中海贫血患者中，组织损伤是由氧化应激引起的，而氧化应激是由于体内铁的积累所致。本研究旨在确定补充锌和维生素E对重型β地中海贫血患者抗氧化状态的影响。
本双盲随机临床试验对120名18岁以上的β地中海贫血患者进行。患者被随机分为四组。前一组给予锌（50毫克/天）补充剂，后一组给予维生素E（400毫克/天）补充剂。第三组给予相同剂量的两种补充剂。第四组（对照组）不给予补充剂。在研究开始和结束时测量补充剂对血清锌、维生素E、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、总抗氧化能力（TAC）和体重指数（BMI）的影响。
第1组和第3组的血清锌水平显著升高（分别为P<0.007和P<0.005）。第2组和第3组的血清维生素E水平也显著升高（P<0.001）。第1、2和3组的平均GPX活性显著降低（分别为P<0.015、P<0.032和P<0.029）。补充后平均SOD活性和TAC没有显著变化。所有治疗组的BMI均显著增加（P<0.001）。
我们的结果表明，β地中海贫血患者的氧化应激增强，给予选择性抗氧化剂可能预防氧化损伤。
