Olsson A G
Scand J Clin Lab Invest. 1979 May;39(3):229-34. doi: 10.3109/00365517909106098.
A quantitative electrophoretic method has been developed in order to differentiate very low density (VLDL) pre-beta lipoproteins from late pre-beta lipoproteins using starch as a supporting medium. It was possible to obtain a bimodal distribution of lipoprotein lipids from VLDL which on agarose gel electrophoresis had a pre-beta band and a late pre-beta band. Optimal conditions were: ammonium carbonate buffer, mu = 0.025, dialysis prior to electrophoresis. Agarose gel electrophoresis demonstrated that the fast and slow components obtained on starch block electrophoresis corresponded to the pre-beta and late pre-beta band respectively. With increasing migration towards the anode the ratio of cholesterol to triglycerides decreased continuously. It is suggested that the fast triglyceride rich component represent newly secreted VLDL species and the slower component mainly postlipolytic particles. The pre-beta band on agarose gel electrophoresis might represent more newly secreted VLDL than the late pre-beta band. However, it cannot be excluded that part of late pre-beta lipoproteins may be secreted de novo.
已开发出一种定量电泳方法，以便使用淀粉作为支持介质，将极低密度（VLDL）前β脂蛋白与晚期前β脂蛋白区分开来。从VLDL获得脂蛋白脂质的双峰分布是可能的，其在琼脂糖凝胶电泳上有一个前β带和一个晚期前β带。最佳条件为：碳酸铵缓冲液，μ = 0.025，电泳前透析。琼脂糖凝胶电泳表明，在淀粉块电泳上获得的快速和慢速成分分别对应于前β带和晚期前β带。随着向阳极迁移的增加，胆固醇与甘油三酯的比率持续下降。有人提出，富含甘油三酯的快速成分代表新分泌的VLDL种类，而较慢的成分主要是脂解后颗粒。琼脂糖凝胶电泳上的前β带可能比晚期前β带代表更多新分泌的VLDL。然而，不能排除部分晚期前β脂蛋白可能是重新分泌的。
