Manzato E, Pagnan A, Ziron L, Gasparotto A, Braggion M
Arteriosclerosis. 1984 Nov-Dec;4(6):598-603. doi: 10.1161/01.atv.4.6.598.
Two electrophoretic populations of very low density lipoprotein are frequently observed in both normal and hyperlipidemic human sera. This gives the appearance of two pre-beta bands, called the "double pre-beta lipoprotein phenomenon." The slow and fast bands forming the double pre-beta lipoprotein were isolated by ultracentrifugation in the zonal rotor under rate flotation conditions. On the basis of the effluent position, the slow band showed a lower flotation coefficient than the fast one. The lipid and apoprotein composition of the two very low density lipoprotein populations isolated by zonal ultracentrifugation was in close agreement with those obtained by preparative agarose gel electrophoresis. The slow and fast pre-beta fractions had the same triglyceride fatty acid composition. Both fractions contained only higher molecular weight apo B-100, while the slow fraction was relatively enriched in apo E and apo C-III. Since the slow pre-beta fraction showed the typical properties of the remnant particle, studying this fraction could clarify the possible relationship between remnant lipoproteins and atherogenesis. Zonal rotor ultracentrifugation may be useful to characterize remnant particles in normal and hyperlipidemic subjects.
在正常人和高脂血症患者的血清中，经常可观察到两种电泳性质的极低密度脂蛋白。这就出现了两条前β带，称为“双前β脂蛋白现象”。在速率浮选条件下，通过区带转子超速离心法分离出构成双前β脂蛋白的慢带和快带。根据流出位置，慢带的浮选系数低于快带。通过区带超速离心法分离得到的两种极低密度脂蛋白群体的脂质和载脂蛋白组成，与通过制备性琼脂糖凝胶电泳得到的结果非常一致。慢前β组分和快前β组分具有相同的甘油三酯脂肪酸组成。两个组分都只含有较高分子量的载脂蛋白B-100，而慢组分相对富含载脂蛋白E和载脂蛋白C-III。由于慢前β组分表现出残余颗粒的典型特性，对该组分的研究可能有助于阐明残余脂蛋白与动脉粥样硬化发生之间的可能关系。区带转子超速离心法可能有助于对正常人和高脂血症患者的残余颗粒进行表征。
