一些金属离子对虹鳟鱼红细胞谷胱甘肽 S-转移酶的影响：一项体外研究。
Effects of some metal ions on rainbow trout erythrocytes glutathione S-transferase enzyme: an in vitro study.
机构信息
Agri Ibrahim Cecen University , Health Services Vocational School TR-04100, Agri , Turkey.
出版信息
J Enzyme Inhib Med Chem. 2013 Dec;28(6):1261-6. doi: 10.3109/14756366.2012.729829. Epub 2012 Oct 12.
Glutathione S-transferase enzyme (GST) (EC 2.5.1.18) was purified from rainbow trout erythrocytes, and some characteristics of the enzyme and effects of some metal ions on enzyme activity were investigated. For this purpose, erythrocyte glutathione S-transferase enzyme which has 16.54 EU/mg protein specific activities was purified 11,026-fold by glutathione-agarose affinity chromatography with a yield of 59%. Temperature was kept under control (+4°C) during purification. Enzyme purification was checked by performing SDS-PAGE. Optimal pH, stable pH, optimal temperature, and K(M) and Vmax values for GSH and 1-chloro-2, 4-dinitrobenzene (CDNB) were also determined for the enzyme. In addition, IC50 values, Ki constants and the type of inhibition were determined by means of Line-Weaver-Burk graphs obtained for such inhibitors as Ag(+); Cd(2+), Cr(2+) and Mg(2+).
谷胱甘肽 S-转移酶（GST）（EC 2.5.1.18）从虹鳟鱼的红细胞中被分离出来，研究了该酶的一些特性以及一些金属离子对酶活性的影响。为此，通过谷胱甘肽琼脂糖亲和层析将红细胞谷胱甘肽 S-转移酶酶纯化了 11026 倍，比活为 16.54 EU/mg 蛋白，收率为 59%。在纯化过程中保持温度在（+4°C）控制之下。通过 SDS-PAGE 检查酶的纯化情况。还确定了酶的最佳 pH 值、稳定 pH 值、最佳温度以及 GSH 和 1-氯-2,4-二硝基苯（CDNB）的 K(M)和 Vmax 值。此外，通过获得的 Line-Weaver-Burk 图谱，用这些抑制剂如 Ag(+)、Cd(2+)、Cr(2+)和 Mg(2+)，确定了 IC50 值、Ki 常数和抑制类型。
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