Department of Plant Physiology, Institute of Biology, University of Leipzig, Johannisallee 23, D-04103 Leipzig, Germany.
Curr Opin Biotechnol. 2013 Feb;24(1):88-94. doi: 10.1016/j.copbio.2012.09.008. Epub 2012 Oct 8.
Estimation of growth potential in a complex community is a great challenge in biotechnical processes and environmental water quality control. Recently it has been shown that the macromolecular structure is a good indicator for the growth potential of phytoplankton cells. A functional understanding of natural phytoplankton communities requires a community analysis by means of single particles technologies. However, conventional biochemical methods are not sensitive enough to determine the macromolecular composition of a single cell or cell aggregates. This problem can be resolved by Fourier transform infrared (FTIR) spectroscopy, which delivers results similar to biochemical analysis with a much smaller sample size. The combined approach of flow cytometric analysis with subcommunity sorting and subsequent FTIR-analysis offers new perspectives for the understanding of community functioning and process optimization.
在生物技术过程和环境水质控制中，对复杂群落中的生长潜力进行估计是一个巨大的挑战。最近表明，大分子结构是浮游植物细胞生长潜力的良好指标。要想对自然浮游植物群落有功能上的了解，就需要通过单颗粒技术对群落进行分析。然而，常规的生化方法不够灵敏，无法确定单细胞或细胞聚集体的大分子组成。傅里叶变换红外（FTIR）光谱可以解决这个问题，它提供的结果与生化分析类似，但所需的样本量要小得多。流式细胞分析与亚群分选相结合，然后进行 FTIR 分析的方法为了解群落功能和优化过程提供了新的视角。
