College of Animal Science and Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, Jilin 130062, China.
Res Vet Sci. 2013 Apr;94(2):346-53. doi: 10.1016/j.rvsc.2012.09.011. Epub 2012 Oct 8.
Two DNA vaccines encoding the envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) alone (pEGFP-GP5) and co-encoding GP5 and swine interleukin-18 (IL-18) proteins (pEGFP-IL18-GP5), were constructed and comparatively evaluated for their abilities to induce humoral and cellular responses in piglets. Experimental results showed that among all vaccinated groups, the live PRRSV vaccine elicited the highest titre of serum neutralizing antibody and the strongest cell-mediated immune responses, which means that the live PRRSV vaccine is still the first option for the vaccination against PRRSV. Moreover, the piglets inoculated with pEGFP-IL18-GP5 developed significantly higher IFN-γ production response, significantly increased percentages of CD4(+) and CD8(+) T-lymphocytes and significantly higher specific T-lymphocyte proliferation response than the pEGFP-GP5 inoculated pigs (P<0.05). These results demonstrated the positive inductive effect of IL-18 on the activation of cellular immune responses in swines. Therefore, in order to develop a new type vaccine for PRRS prevention and control, co-encoding of GP5 and IL-18 proteins may be a good choice.
两种 DNA 疫苗分别单独（pEGFP-GP5）和共编码猪繁殖与呼吸综合征病毒（PRRSV）包膜蛋白 GP5 和猪白细胞介素-18（IL-18）蛋白（pEGFP-IL18-GP5），构建并比较评估了它们在仔猪中诱导体液和细胞反应的能力。实验结果表明，在所有接种组中，活 PRRSV 疫苗引起的血清中和抗体效价最高，细胞介导的免疫反应最强，这意味着活 PRRSV 疫苗仍然是预防 PRRSV 的首选疫苗。此外，接种 pEGFP-IL18-GP5 的仔猪产生 IFN-γ的能力显著增强，CD4+和 CD8+T 淋巴细胞的百分比显著增加，特异性 T 淋巴细胞增殖反应显著增强，比接种 pEGFP-GP5 的仔猪高（P<0.05）。这些结果表明 IL-18 对猪细胞免疫反应的激活具有积极的诱导作用。因此，为了开发预防和控制 PRRS 的新型疫苗，共编码 GP5 和 IL-18 蛋白可能是一个不错的选择。
