Petrini Stefano, Ramadori Giorgio, Villa Riccardo, Borghetti Paolo, de Angelis Elena, Cantoni Anna Maria, Corradi Attilio, Amici Augusto, Ferrari Maura
Umbria and Marche Experimental Zooprophylaxis Institute, via Gaetano Salvemini 1, Perugia 06126, Italy.
Genetic Immunization Unit, Department of Biology, University of Camerino, Via Camerini 1, Camerino 62032, Italy.
Vaccines (Basel). 2013 Oct 18;1(4):463-80. doi: 10.3390/vaccines1040463.
In veterinary medicine, there have been different experiences with the plasmid DNA vaccination. In this area and with the hypothesis to demonstrate the effectiveness of different plasmids encoding porcine respiratory and reproductive syndrome (PRRS), five DNA vaccines against PRRS were evaluated for their innocuity and efficacy in pigs. Eighteen animals were divided into five groups which were injected with five (A, B, C, D, E) different DNA vaccines. Albeit, none of the proposed vaccines were able to protect the animals against PRRS virus. Only vaccines A and B were able to reduce the clinical signs of the infection. ELISA IgM were detected 30 days after the first vaccination in the pigs injected by Vaccine A or B. ELISA IgG were detected 90 days after the first vaccination in the pigs injected by Vaccine B or C. Neutralizing antibody were detected Post Challenge Days 61 (PCD) in all groups. In the pigs inoculated with Vaccine C, IFN-g were detected 90 days after first vaccination, and after challenge exposure they increased. In the other groups, the IFN-g were detected after challenge infection. Pigs injected with each of the vaccines A, B, C, D and E showed a significantly higher level of CD4(-)CD8⁺ lymphocytes (p < 0.001) after infection in comparison with their controls.
在兽医学中，质粒DNA疫苗接种有着不同的经验。在该领域，并基于证明不同编码猪繁殖与呼吸综合征（PRRS）的质粒有效性的假设，对五种针对PRRS的DNA疫苗在猪身上的无害性和有效性进行了评估。18只动物被分为五组，分别注射五种（A、B、C、D、E）不同的DNA疫苗。然而，所提出的疫苗均未能保护动物免受PRRS病毒感染。只有疫苗A和B能够减轻感染的临床症状。在首次接种疫苗30天后，在注射疫苗A或B的猪中检测到ELISA IgM。在首次接种疫苗90天后，在注射疫苗B或C的猪中检测到ELISA IgG。在攻毒后第61天（PCD）在所有组中检测到中和抗体。在接种疫苗C的猪中，首次接种疫苗90天后检测到IFN - g，攻毒后其水平升高。在其他组中，IFN - g在攻毒感染后检测到。与对照组相比，注射疫苗A、B、C、D和E的猪在感染后CD4(-)CD8⁺淋巴细胞水平显著更高（p < 0.001）。
