Gutiérrez-Mercado Yanet K, Cañedo-Dorantes Luis, Gómez-Pinedo Ulises, Serrano-Luna Gregorio, Bañuelos-Pineda Jacinto, Feria-Velasco Alfredo
Laboratory of Cellular Neurobiology, Biological and Agricultural Sciences University Center (CUCBA), University of Guadalajara, Guadalajara, Mexico.
Bioelectromagnetics. 2013 Feb;34(2):145-55. doi: 10.1002/bem.21757. Epub 2012 Oct 11.
It has been demonstrated that the exposure of biological systems to magnetic fields (MFs) can produce several beneficial effects: tissue recovery in chronic wounds, re-establishment of blood circulation after tissue ischemia or in necrotic tissues, improvement after epileptic episodes, angiogenesis, etc. In the current study, the effects of extremely low frequency (ELF) MF on the capillaries of some circumventricular organs (CVOs) are demonstrated; a vasodilator effect is reported as well as an increase in their permeability to non-liposoluble substances. For this study, 96 Wistar male rats (250 g body mass) were used and divided into three groups of 32 rats each: a control group (no treatment); a sham ELF-MF group; and an experimental group subjected to ELF-MF (120 Hz harmonic waves and 0.66 mT, root mean square) by the use of Helmholtz coils. All animals were administered colloidal carbon (CC) intravenously to study, through optical and transmission electron microscopy, the capillary permeability in CVOs and the blood-brain barrier (BBB) in brain areas. An increase in capillary permeability to CC was detected in the ELF-MF-exposed group as well as a significant increase in vascular area (capillary vasodilation); none of these effects were observed in individuals of the control and sham ELF-MF groups. It is important to investigate the mechanisms involved in the phenomena reported here in order to explain the effects of ELF-MF on brain vasculature.
已经证明，生物系统暴露于磁场（MFs）会产生多种有益效果：慢性伤口的组织恢复、组织缺血后或坏死组织中的血液循环重建、癫痫发作后的改善、血管生成等。在当前研究中，证明了极低频（ELF）MF对一些室周器官（CVOs）毛细血管的影响；报告了血管舒张作用以及它们对非脂溶性物质通透性的增加。在本研究中，使用了96只Wistar雄性大鼠（体重250克），并将其分为三组，每组32只大鼠：对照组（不进行处理）；假ELF-MF组；以及通过使用亥姆霍兹线圈接受ELF-MF（120赫兹谐波和0.66毫特斯拉，均方根）的实验组。所有动物均静脉注射胶体碳（CC），以通过光学和透射电子显微镜研究CVOs中的毛细血管通透性以及脑区的血脑屏障（BBB）。在暴露于ELF-MF的组中检测到毛细血管对CC的通透性增加以及血管面积的显著增加（毛细血管舒张）；在对照组和假ELF-MF组的个体中未观察到这些效应。为了解释ELF-MF对脑血管系统的影响，研究此处报道现象所涉及的机制很重要。
