Centre of Ecology and Hydrology, MacLean Building, Benson Lane, Crowmarsh Gifford, Wallingford OX10 8BB, United Kingdom.
Environ Pollut. 2013 Jan;172:200-7. doi: 10.1016/j.envpol.2012.09.005. Epub 2012 Oct 11.
Exposures of Lumbricus rubellus to a series of arsenic concentrations in soil were used to assess life-stage (juvenile, adult) and genotype specific sensitivities, to calculate population growth rate (λ) and to assess patterns of As accumulation. Significant mortality was seen in juveniles at 125 mg/kg As, while growth and maturation was affected from 36 mg/kg and above. In adults, cocoon production at the highest concentration (125 mg/kg) was significantly reduced. Phylogenetic analysis was performed by comparison of mitochondrial sequences to establish genotypic variation among juveniles. Three clades with more than 7.5% divergent were described, with 70% of earthworms belonging to a single clade. Date of and mass at maturation was significantly different between clades, but clades were not differentially As sensitive. Parameter λ was reduced at 36 mg/kg As and was negative at 125 mg/kg As, suggesting impacts and population stability and potential extinction at environmentally relevant concentrations.
用赤子爱胜蚓暴露于一系列土壤砷浓度的方法来评估生命阶段（幼体、成体）和基因型特异性敏感性，计算种群增长率（λ）并评估砷积累模式。在 125mg/kg As 时，幼体出现显著死亡率，而在 36mg/kg 及以上时，生长和成熟受到影响。在成体中，最高浓度（125mg/kg）的茧产量显著减少。通过比较线粒体序列进行系统发育分析，以确定幼体中的基因型变异。描述了三个具有超过 7.5%差异的分支，其中 70%的蚯蚓属于单个分支。成熟的日期和质量在分支之间有显著差异，但分支对砷的敏感性没有差异。参数 λ 在 36mg/kg As 时降低，在 125mg/kg As 时为负，表明在环境相关浓度下存在影响和种群稳定性以及潜在灭绝的风险。
