Cossar D, Bell J, Strange R, Jones M, Sandison A, Hume R
Department of Pathology, University of Edinburgh, U.K.
Biochim Biophys Acta. 1990 Feb 9;1037(2):221-6. doi: 10.1016/0167-4838(90)90171-b.
Polyclonal antisera to the alpha and pi isoenzymes of glutathione S-transferase have been used in immunohistochemical studies of developing human lung. In utero expression of the pi set was down-regulated in distal airway cells and the first appearance of pi-negative cells coincided with phenotypic differentiation. In contrast, in the early phase of fetal lung organ culture pi isoenzyme was detected in all differentiated epithelial cells and only as culture progressed did focal negativity develop. The alpha set showed no developmental changes in utero or in organ culture.
针对谷胱甘肽S-转移酶α和π同工酶的多克隆抗血清已用于发育中的人类肺脏的免疫组织化学研究。在子宫内，π同工酶在远端气道细胞中的表达下调，π阴性细胞的首次出现与表型分化同时发生。相比之下，在胎儿肺器官培养的早期阶段，在所有分化的上皮细胞中都检测到了π同工酶，只有随着培养的进行才出现局灶性阴性。α同工酶在子宫内或器官培养中均未显示出发育变化。
