人肺中谷胱甘肽S-转移酶和谷胱甘肽过氧化物酶活性的发展。

The development of glutathione S-transferase and glutathione peroxidase activities in human lung.

作者信息

Fryer A A, Hume R, Strange R C

出版信息

Biochim Biophys Acta. 1986 Oct 1;883(3):448-53. doi: 10.1016/0304-4165(86)90283-7.

Abstract

The development of glutathione S-transferase and glutathione peroxidase activities has been studied in human lung cytosols. Whilst no clear change in glutathione peroxidase activity was identified, expression of the acidic glutathione S-transferase isoenzyme decreased markedly after 15 weeks of gestation so that at birth the level of activity of this isoenzyme was only about 20% of that in samples obtained during the first trimester. Basic glutathione S-transferase isoenzymes were weakly expressed during development and usually comprised less than 10% of cytosolic activity. Ion-exchange studies identified several basic isoenzymes that may correspond to the alpha, beta, gamma, delta and epsilon set previously identified in liver. Weak expression of apparently near-neutral isoenzymes was also detected; they were detected in only a few cytosols.

摘要

已对人肺胞质溶胶中谷胱甘肽S-转移酶和谷胱甘肽过氧化物酶活性的发育情况进行了研究。虽然未发现谷胱甘肽过氧化物酶活性有明显变化，但酸性谷胱甘肽S-转移酶同工酶的表达在妊娠15周后显著下降，以至于出生时该同工酶的活性水平仅约为孕早期所取样本中活性水平的20%。碱性谷胱甘肽S-转移酶同工酶在发育过程中表达较弱，通常占胞质溶胶活性的比例不到10%。离子交换研究确定了几种碱性同工酶，它们可能与先前在肝脏中鉴定出的α、β、γ、δ和ε组相对应。还检测到明显接近中性的同工酶表达较弱；仅在少数胞质溶胶中检测到它们。