State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (MOE), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
Mol Biol Rep. 2013 Feb;40(2):1009-19. doi: 10.1007/s11033-012-2142-6. Epub 2012 Oct 14.
QDs have large scale application in many important areas with potential of unintentional exposure to the environment or organism during processing of a nanotechnology containing product's life cycle. In this paper, two classical electrochemical methods, cyclic voltammetry and electrochemical impedance spectroscopy were applied to investigate the influence of particle sizes of CdTe QDs on their toxicity targeted to human serum albumin (HSA) under simulative physiological conditions. The results show that the toxicity of yellow emitting QDs (YQDs) on HSA is slightly stronger than that of the green-emitting (GQDs) and red-emitting QDs (RQDs). We also compared these two classical electrochemical methods with the traditional fluorescence spectroscopy through the above results. The electrochemical methods may be more accurate and comprehensive to investigate the toxicity of QDs at the biomacromolecular level under certain conditions, though fluorescence spectroscopy is simpler and more sensitive.
量子点在许多重要领域有大规模的应用，在含有纳米技术的产品生命周期中处理过程中，可能会无意暴露于环境或生物体中。在本文中，两种经典的电化学方法，循环伏安法和电化学阻抗谱法，被应用于在模拟生理条件下，研究 CdTe 量子点的粒径对其与人血清白蛋白（HSA）毒性的影响。结果表明，在 HSA 上，发射黄色光的量子点（YQDs）的毒性略强于发射绿光的量子点（GQDs）和发射红光的量子点（RQDs）。我们还通过上述结果将这两种经典电化学方法与传统荧光光谱法进行了比较。虽然荧光光谱法更简单、更灵敏，但在某些条件下，电化学方法可能更准确、更全面地研究量子点在生物大分子水平上的毒性。
