Parker C E, Verma S, Tomer K B, Reed R L, Buhler D R
Laboratory of Molecular Biophysics, NIEHS, Research Triangle Park, North Carolina 27709.
Biomed Environ Mass Spectrom. 1990 Jan;19(1):1-12. doi: 10.1002/bms.1200190102.
A series of Senecio alkaloid and alkaloid N-oxide standards has been analyzed using positive and negative ion thermospray liquid chromatography/mass spectrometry (LC/MS) with an ammonium acetate-containing mobile phase. On-line separations of pyrrolizidine alkaloids from extracts of Senecio jacobaea (tansy ragwort) and Senecio vulgaris (common groundsel) were done using an ammonium hydroxide-containing mobile phase. All of the alkaloids known to be present in the extracts were detected by ammonium hydroxide thermospray LC/MS, as well as many other components which may be as-yet-unidentified alkaloids.
使用含醋酸铵的流动相，通过正离子和负离子热喷雾液相色谱/质谱联用（LC/MS）对一系列千里光生物碱和生物碱N-氧化物标准品进行了分析。使用含氢氧化铵的流动相，对来自欧洲千里光（艾菊）和普通千里光（千里光）提取物中的吡咯里西啶生物碱进行了在线分离。通过氢氧化铵热喷雾LC/MS检测到了提取物中已知存在的所有生物碱，以及许多其他可能尚未鉴定的生物碱成分。
