利用激光解吸傅里叶变换质谱法对光诱导嘧啶环丁烷二聚体进行表征。
Characterization of photo-induced pyrimidine cyclobutane dimers by laser desorption Fourier transform mass spectrometry.
作者信息
Hettich R L, Buchanan M V, Ho C H
机构信息
Analytical Chemistry Division, Oak Ridge National Laboratory, Tennessee 37831-6120.
出版信息
Biomed Environ Mass Spectrom. 1990 Feb;19(2):55-62. doi: 10.1002/bms.1200190202.
Laser desorption Fourier transform mass spectrometry was used to characterize the cis-syn cyclobutane photodimers of uracil-uracil, uracil-thymine and thymine-thymine. This soft ionization technique generated [M-H]- ions as well as some fragment ions. Investigation of the laser desorption process indicated that gas-phase dimerization reactions do not occur for pyrimidine monomers and dimers under these experimental conditions. Collisional dissociation of the [M-H]- ions provided structural information for the pyrimidine rings of the dimers. The fragment ions observed in the collisional dissociation spectra of these cyclobutane dimers suggested rearrangement of the [M-H]- parent ions to a macrocycle prior to dissociation.
激光解吸傅里叶变换质谱法用于表征尿嘧啶 - 尿嘧啶、尿嘧啶 - 胸腺嘧啶和胸腺嘧啶 - 胸腺嘧啶的顺式 - 顺环丁烷光二聚体。这种软电离技术产生了[M-H]-离子以及一些碎片离子。对激光解吸过程的研究表明，在这些实验条件下，嘧啶单体和二聚体不会发生气相二聚反应。[M-H]-离子的碰撞解离提供了二聚体嘧啶环的结构信息。在这些环丁烷二聚体的碰撞解离光谱中观察到的碎片离子表明，[M-H]-母离子在解离之前重排为大环。
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