Itoh Takayuki, Kushiro Tetsuo, Fujii Isao
Research Institute for Bioscience Products and Fine Chemicals, Ajinomoto Co., Kawasaki, Kanagawa, Japan.
Methods Mol Biol. 2012;944:175-82. doi: 10.1007/978-1-62703-122-6_12.
Expression of multiple genes involved in a particular metabolic pathway in a heterologous host facilitates the study of fungal secondary metabolite biosynthesis and production of useful compounds. Two plasmids with different selection markers, argB and the pyrithiamine resistance marker, are used to transform Aspergillus oryzae allowing for expression of up to three genes simultaneously.
在异源宿主中表达参与特定代谢途径的多个基因，有助于研究真菌次级代谢产物的生物合成以及有用化合物的生产。携带不同选择标记（argB和抗硫胺素标记）的两种质粒用于转化米曲霉，从而可同时表达多达三个基因。
