一种用于工业3.042的双选择标记转化系统
A Dual Selection Marker Transformation System Using for the Industrial 3.042.
作者信息
Sun Yunlong, Niu Yali, He Bin, Ma Long, Li Ganghua, Tran Van-Tuan, Zeng Bin, Hu Zhihong
机构信息
Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, P.R. China.
Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization (Hubei Normal University), 435002, Huangshi, P.R. China.
出版信息
J Microbiol Biotechnol. 2019 Feb 28;29(2):230-234. doi: 10.4014/jmb.1811.11027.
Currently, the genetic modification of is mainly dependent on protoplastmediated transformation (PMT). In this study, we established a dual selection marker system in an industrial 3.042 strain by using -mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of 3.042.
目前，[具体生物名称]的基因改造主要依赖于原生质体介导转化（PMT）。在本研究中，我们通过农杆菌介导转化（ATMT）在一株工业用3.042菌株中建立了双选择标记系统。我们首先构建了一个尿苷/尿嘧啶营养缺陷型3.042菌株和一个抗嘧啶硫胺素（PT）的二元载体。然后，我们以尿苷/尿嘧啶营养缺陷型和PT抗性基因作为选择标记建立了ATMT系统。最后，开发了一种双选择标记ATMT系统。本研究证明了一种用于3.042基因操作的有用的双选择标记转化系统。
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