University of Tasmania, ACROSS and School of Chemistry, Hobart, Australia.
Electrophoresis. 2012 Nov;33(21):3166-72. doi: 10.1002/elps.201200141. Epub 2012 Oct 12.
ITP with indirect fluorescence detection (IFD) was introduced three decades ago. Despite this fact, the method has never become widely adopted. The main aim of this work was to utilize the ITP-IFD for the separation of carboxylic acids by using a commercially available, portable, microfluidic chip electrophoresis system. On the 16.8-mm effective length separation channel, a maximum of eight carboxylic acids could be separated, with LOD values in a range from 0.12 to 0.4 mM. The commercial chips used for all experiments have multichannel structures important for analysis of more than one sample per a chip in case of standard use. This multichannel structure was used to investigate the possibility of multiple sample loading for ITP separation. Application of ITP-IFD was investigated for analysis of benzoate in diet soft drinks and the results were in good agreement with results of a CE method.
间接免疫荧光检测法（IFD）在三十年前就已问世。尽管如此，该方法却从未得到广泛应用。本工作的主要目的是利用商业上可用的、便携式的微流控芯片电泳系统，通过 ITP-IFD 分离羧酸。在 16.8 毫米有效长度的分离通道中，最多可分离八种羧酸，LOD 值范围为 0.12 至 0.4 mM。所有实验中使用的商业芯片均具有多通道结构，这对于标准使用情况下每个芯片分析多个样品非常重要。该多通道结构用于研究用于 ITP 分离的多个样品加载的可能性。我们还研究了 ITP-IFD 在分析饮食软饮料中的苯甲酸盐中的应用，其结果与 CE 方法的结果非常吻合。
