接触性皮炎的实验模型：用游离和纳米包封氯倍他索处理的 Wistar 大鼠的氧化谱评估。
An experimental model of contact dermatitis: evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol.
机构信息
Departamento de Química, Laboratório de Enzimologia Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, Brasil.
出版信息
Redox Rep. 2012;17(5):206-13. doi: 10.1179/1351000212Y.0000000024.
OBJECTIVE
An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue.
METHODS
Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO(4). Groups 3 and 4 were sensitized with 5% NiSO(4) and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates.
RESULTS
A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP.
DISCUSSION
Our results suggest that both NiSO(4) sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.
目的
采用接触性皮炎（CD）实验动物模型，研究游离态和纳米封装丙酸氯倍他索对皮肤和肝脏组织氧化谱的影响。
方法
将雌性 Wistar 大鼠分为 6 组，每组 8 只。第 1 组为对照组（C），用固体凡士林致敏。第 2 组（CD）用 5%硫酸镍致敏。第 3 组和第 4 组用 5%硫酸镍致敏，分别用游离（FC）和纳米封装（NC）丙酸氯倍他索（0.42mg/g）每天处理 5 天。第 5 组在第 1、3 和 5 天用纳米封装丙酸氯倍他索（0.42mg/g）处理（C135），第 6 组每天用含有空纳米颗粒（NP）的水凝胶处理 5 天。测量肝匀浆中的硫代巴比妥酸反应物（TBARS）、羰基水平、非蛋白巯基（NPSH）和过氧化氢酶活性。
结果
CD 组和 NP 组的 TBARS、NPSH 和过氧化氢酶活性水平均显著升高。
讨论
我们的结果表明，NiSO4 致敏和 NP 给药均诱导细胞脂质氧化，并激活抗氧化酶过氧化氢酶以保护免受这种损伤。这些结果还表明，游离态和纳米封装丙酸氯倍他索的每日治疗，以及每隔一天用纳米封装丙酸氯倍他索治疗，能够预防这些氧化还原改变，并防止组织学损伤。
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