Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Skin Pharmacol Physiol. 2011;24(3):166-74. doi: 10.1159/000323273. Epub 2011 Jan 26.
To evaluate the effect of cationic coating of polymeric nanocapsules in sunscreen formulations on the in vitro skin penetration of benzophenone-3.
Benzophenone-3-loaded nanocapsules were prepared by the interfacial deposition of poly(ε-caprolactone) and coated by using a chitosan solution. The nanoparticles were characterized and incorporated in hydrogels. The presence of nanoparticles in hydroxyethyl cellulose gels was observed by transmission electron microscopy and photon correlation spectroscopy. Penetration studies were carried out using Franz cells with porcine skin membranes.
Benzophenone-3-loaded chitosan-coated nanocapsules presented a mean size of 202 ± 7 nm and positive zeta potential (+21 ± 1 mV), while these values for the uncoated nanocapsules were 175 ± 1 nm and -8 ± 1 mV. Penetration profiles showed that a higher amount of benzophenone-3 remained at the skin surface and a lower amount was found in the receptor compartment after the application of the formulation containing chitosan-coated nanocapsules compared to a formulation containing its free form.
Hydrogel containing benzophenone-3 chitosan-coated nanocapsules represents an innovative formulation to overcome limitations of sunscreen daily use.
评估聚合物纳米胶囊阳离子涂层在防晒配方中对二苯甲酮-3 体外经皮渗透的影响。
采用界面聚合法制备载有二苯甲酮-3 的纳米胶囊，并采用壳聚糖溶液对其进行涂层。对纳米颗粒进行了表征，并将其掺入水凝胶中。通过透射电子显微镜和光子相关光谱观察到纳米颗粒在羟乙基纤维素凝胶中的存在。使用Franz 细胞和猪皮膜进行渗透研究。
载有二苯甲酮-3 的壳聚糖涂层纳米胶囊的平均粒径为 202 ± 7nm，表面带有正电荷（+21 ± 1mV），而未涂层纳米胶囊的粒径为 175 ± 1nm，表面带有负电荷（-8 ± 1mV）。渗透曲线表明，与含有二苯甲酮-3 游离形式的配方相比，含有壳聚糖涂层纳米胶囊的配方在应用后，有更多的二苯甲酮-3 停留在皮肤表面，而在受体室中的含量较低。
含有二苯甲酮-3 壳聚糖涂层纳米胶囊的水凝胶是一种克服防晒霜日常使用局限性的创新配方。
