Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Tunis, Tunisie.
J Physiol Pharmacol. 2012 Aug;63(4):381-8.
High garlic dose could exert adverse health properties and grape seed and skin extract (GSSE) exhibit a variety of beneficial effects, even at high dose. In the present study we evaluated the toxic effect of high garlic dose treatment on antioxidant status of the blood compartment and the protective effect of GSSE. Rats were intraperitoneally (i.p.) administered either with garlic extract (5 g/kg bw) or GSSE (500 mg/kg bw) or a combination of garlic and GSSE at the same doses daily during one month. Plasma parameters and erythrocytes antioxidant status were evaluated. Data confirmed that high garlic dose induced anemia and a pro-oxidative state into erythrocytes characterized by increased malondialdehyde (MDA), carbonyl protein and antioxidant enzyme activities as catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Garlic also elevated intracellular hydrogen peroxide (H(2)O(2)) and free iron whereas GSSE treatment counteracted almost all garlic deleterious effects. In conclusion, high garlic dose induced a pro-oxidative state into erythrocytes via the Fenton reaction between H(2)O(2) and free iron, and GSSE exerted antioxidant properties.
高剂量大蒜可能会对健康产生不良影响，而葡萄籽和皮提取物（GSSE）即使在高剂量下也表现出多种有益作用。在本研究中，我们评估了高剂量大蒜处理对血液区室抗氧化状态的毒性作用以及 GSSE 的保护作用。大鼠通过腹膜内（i.p.）给予大蒜提取物（5 g/kg bw）或 GSSE（500 mg/kg bw）或以相同剂量的大蒜和 GSSE 的组合每天处理一个月。评估了血浆参数和红细胞抗氧化状态。数据证实，高剂量大蒜会导致贫血和红细胞的氧化应激状态，其特征是丙二醛（MDA）、羰基蛋白和抗氧化酶活性（CAT、POD 和 SOD）增加。大蒜还增加了细胞内过氧化氢（H2O2）和游离铁，而 GSSE 处理几乎抵消了大蒜的所有有害作用。总之，高剂量大蒜通过 H2O2 和游离铁之间的 Fenton 反应诱导红细胞的氧化应激状态，而 GSSE 则表现出抗氧化特性。
