Mokni Meherzia, Hamlaoui Sonia, Kadri Safwen, Limam Ferid, Amri Mohamed, Marzouki Lamjed, Aouani Ezzedine
Laboratoire de Neurophysiologie Fonctionnelle et Pathologies, Département des Sciences Biologiques, Faculté des Sciences de Tunis, Campus Universitaire El Manar II-2092 Tunis, Tunisie.
Laboratoire des Substances Bioactives, Centre de Biotechnologie, Technopole Borj-Cedria, Hammam-Lif, Tunisie.
Pak J Pharm Sci. 2016 May;29(3):961-8.
The study investigated the protective effect of grape seed and skin extract (GSSE) against doxorubicin-induced renal toxicity in healthy rats. Animals were treated with GSSE or not (control), for 8 days, administered with doxorubicin (20mg/kg) in the 4th day, and renal function as well as oxidative stress parameters were evaluated. Data showed that doxorubicin induced renal toxicity by affecting renal architecture and plasma creatinine. Doxorubicin also induced an oxidative stress characterized by an increase in malondialdehyde (MDA), calcium and H(2)O(2) and a decrease in catalase (CAT) and superoxide dismutase (SOD). Unexpectedly doxorubicin increased peroxidase (POD) and decreased carbonyl protein and plasma urea. Treatment with GSSE counteracted almost all adverse effects induced by doxorubicin. Data suggest that doxorubicin induced an oxidative stress into rat kidney and GSSE exerted antioxidant properties, which seem to be mediated by the modulation of intracellular calcium.
该研究调查了葡萄籽和葡萄皮提取物（GSSE）对阿霉素诱导的健康大鼠肾毒性的保护作用。动物接受GSSE处理或不处理（对照组），持续8天，在第4天给予阿霉素（20mg/kg），并评估肾功能以及氧化应激参数。数据显示，阿霉素通过影响肾脏结构和血浆肌酐诱导肾毒性。阿霉素还诱导了以丙二醛（MDA）、钙和H₂O₂增加以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）减少为特征的氧化应激。出乎意料的是，阿霉素增加了过氧化物酶（POD），并降低了羰基蛋白和血浆尿素。GSSE处理抵消了阿霉素诱导的几乎所有不良反应。数据表明，阿霉素在大鼠肾脏中诱导了氧化应激，而GSSE发挥了抗氧化特性，这似乎是由细胞内钙的调节介导的。
