College of Life Sciences, Central China Normal University, Luoyu Road 152, Wuhan City, 430079, Hubei Province, People's Republic of China.
Cytotechnology. 2013 May;65(3):425-35. doi: 10.1007/s10616-012-9496-4. Epub 2012 Oct 16.
Development of insect resistance to Bacillus thuringiensis (Bt) toxins threatens the sustained successful application of Bt-based biological control tactics. Multi-mechanisms of resistance have been proposed, such as alteration of toxin-binding proteins, changes of proteases in midgut and so on. The other responses of the Cry1Ac-selected insects might also contribute to the evolution of resistance. Here, the Cry1Ac-selected Trichoplusia ni TnH5 cells with high resistance were subjected to analysis of proteome and the differentially expressed proteins were identified using mass spectrometry. The differential proteins included transporter, molecular chaperon, structural molecules and many other molecules involved in protein metabolism, signal transduction, nucleotide binding, lipid biosynthesis, carbohydrates metabolism and energy production, suggesting that a complex mechanisms involved in the development of insect resistance to Bt Cry1Ac toxins at cellular levels. The decrease of protein synthesis, changes of signal transduction, more rapid energy production, the enhanced lipid synthesis and the decline of possible Cry1Ac-binding proteins in cytoplasm and other events might contribute to the development of resistance in the selected cells. Our results provide some new cues for understanding the mechanism of Bt resistance.
昆虫对苏云金芽孢杆菌（Bt）毒素的抗药性发展威胁到 Bt 生物防治策略的持续成功应用。已经提出了多种抗药性机制，例如毒素结合蛋白的改变、中肠蛋白酶的变化等。Cry1Ac 选择的昆虫的其他反应也可能有助于抗药性的进化。在这里，用 Cry1Ac 选择的具有高抗性的斜纹夜蛾 TnH5 细胞进行蛋白质组分析，并使用质谱鉴定差异表达的蛋白质。差异蛋白包括转运蛋白、分子伴侣、结构分子和许多其他参与蛋白质代谢、信号转导、核苷酸结合、脂质生物合成、碳水化合物代谢和能量产生的分子，表明在细胞水平上，昆虫对 Bt Cry1Ac 毒素的抗药性发展涉及复杂的机制。蛋白质合成的减少、信号转导的改变、更快的能量产生、脂质合成的增强以及细胞质中可能的 Cry1Ac 结合蛋白的减少和其他事件可能有助于选择细胞的抗药性发展。我们的结果为理解 Bt 抗药性机制提供了一些新的线索。
