Advanced Ceramics Research Center, Nagoya Institute of Technology, 3-101-1 Honmachi, Tajimi, Gifu 507-0033, Japan.
Microsc Res Tech. 2013 Jan;76(1):66-71. doi: 10.1002/jemt.22137. Epub 2012 Oct 16.
The method to observe the exact morphology of swelled seaweed as an example of biological material by field emission scanning electron microscopy (FE-SEM) with the aid of hydrophilic ionic liquid (IL); 1-butyl-3-methylimidazolium tetrafluoroborate is reported. Seaweed was first swelled in 3.5% NaCl solution and then treated with the IL and water mixture in 1:7 weight ratios and centrifuged to remove the excess IL solution. Thus treated seaweed maintained its morphology even at high magnification and did not show drying in the FE-SEM chamber. This observation technique might be useful for various kinds of biological materials to be observed under FE-SEM.
本文报道了一种利用场发射扫描电子显微镜（FE-SEM）观察生物材料膨胀海藻的精确形态的方法，该方法借助亲水性离子液体（IL），即 1-丁基-3-甲基咪唑四氟硼酸盐。海藻首先在 3.5%的 NaCl 溶液中膨胀，然后与 1:7 重量比的 IL 和水混合物处理，并离心去除多余的 IL 溶液。因此，处理后的海藻即使在高倍放大下也能保持其形态，并且在 FE-SEM 腔中不会出现干燥现象。这种观察技术可能对各种需要在 FE-SEM 下观察的生物材料有用。
