关于DNA结构序列依赖性的一则注释。

A note on sequence-dependence of DNA structure.

作者信息

Galat A

机构信息

Department of Chemistry, Harvard University, Cambridge, MA 02138.

出版信息

Eur Biophys J. 1990;17(6):331-42. doi: 10.1007/BF00258382.

DOI:10.1007/BF00258382

PMID:2307141

Abstract

A circular dichroism study was conducted on the solution structure of several different oligonucleotides, whose X-ray structures have been solved. It is suggested that in aqueous solution the oligonucleotides can form structures that maintain geometrical elements which are typical of B-DNA, A-DNA, and their intermediate forms. It is shown that 5'GGATGGGAG:5'CTCCCATCC, which forms an A-DNA helix in the crystal state (McCall et al. 1986), in aqueous solution maintains an A-DNA like structure at temperatures below 10 degrees C. At temperatures between 10 degrees C and 25 degrees C it shows a tendency to form an intermediate structure between A-DNA and B-DNA. Also, it is shown that TFE does not cause a transition from B-DNA to A-DNA helix in short DNA fragments, but instead disrupts the helix.

摘要

对几种不同寡核苷酸的溶液结构进行了圆二色性研究，这些寡核苷酸的X射线结构已得到解析。研究表明，在水溶液中，寡核苷酸可以形成维持B-DNA、A-DNA及其中间形式典型几何元素的结构。结果显示，5'GGATGGGAG:5'CTCCCATCC在晶体状态下形成A-DNA螺旋（麦考尔等人，1986年），在水溶液中，在低于10摄氏度的温度下保持类似A-DNA的结构。在10摄氏度至25摄氏度之间的温度下，它显示出形成A-DNA和B-DNA之间中间结构的趋势。此外，研究表明，三氟乙醇（TFE）不会导致短DNA片段从B-DNA向A-DNA螺旋转变，反而会破坏螺旋结构。