Wang Pin-Jia, Xie Cheng-Bin
Department of Laboratory Medicine, Chengdu Medical College, Chengdu 610083, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Jun 30;30(3):210-3.
To investigate the relation of Mycoplasma hominis symbiosis and the resistance of Trichomonas vaginalis to metronidazole.
From November 2010 to July 2011, 160 isolates of T. vaginalis were collected from the genital tract secretion of gynecological out-patients at the Sichuan Provincial Hospital for Women and Children. The minimum lethal concentration (MLC) to metronidazole of these isolates was determined by an in vitro sensitivity assay with different concentration gradients of metronidazole (from 1 to 1 024 microg/ml), and M. hominis DNA in T. vaginalis was detected by polymerase chain reaction (PCR) technique with specific 16S rRNA primers. After clearance of M. hominis from the parasites by 32 microg/ml doxycycline, MLC was determined and compared with that before clearance.
MLC of metronidazole in T. vaginalis ranged from 1 to 256 microg/ml, with 61.3% isolates (98/160) ranging from 1 to 8 microg/ml, 26.3% isolates (42/160) ranging from 16 to 32 microg/ml, and 12.5% isolates (20/160) ranging from 64 to 256 microg/ml. 61 isolates were PCR positive for M. hominis DNA in the 160 isolates of T. vaginalis. The M. hominis DNA positive rate was significantly higher in the T. vaginalis isolates with higher MLC than those isolates with lower MLC (P<0.01). However, when M. hominis was cleared by doxycycline from 8 isolates among the 61 ones, no change was observed in sensitivity of the isolates to metronidazole.
M. hominis symbiosis might be associated with the metronidazole-resistance of T. vaginalis. However, it needs direct evidence.
探讨人型支原体共生与阴道毛滴虫对甲硝唑耐药性的关系。
2010年11月至2011年7月，从四川省妇女儿童医院妇科门诊患者生殖道分泌物中收集160株阴道毛滴虫。采用不同浓度梯度（1至1024μg/ml）的甲硝唑进行体外药敏试验，测定这些分离株对甲硝唑的最低致死浓度（MLC），并用特异性16S rRNA引物通过聚合酶链反应（PCR）技术检测阴道毛滴虫中人型支原体DNA。用32μg/ml强力霉素清除寄生虫中的人型支原体后，测定MLC并与清除前进行比较。
阴道毛滴虫对甲硝唑的MLC范围为1至256μg/ml，其中61.3%（98/160）的分离株MLC为1至8μg/ml，26.3%（42/160）的分离株MLC为16至32μg/ml，12.5%（20/160）的分离株MLC为64至256μg/ml。在160株阴道毛滴虫分离株中，61株人型支原体DNA PCR检测呈阳性。MLC较高的阴道毛滴虫分离株中人型支原体DNA阳性率显著高于MLC较低的分离株（P<0.01）。然而，在用强力霉素清除61株中的8株人型支原体后，这些分离株对甲硝唑的敏感性未观察到变化。
人型支原体共生可能与阴道毛滴虫对甲硝唑的耐药性有关。然而，这需要直接证据。
