School of Clinical Medicine Laboratory, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
Department of Biostatistics, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.
Parasitol Res. 2020 Dec;119(12):4197-4204. doi: 10.1007/s00436-020-06930-x. Epub 2020 Oct 17.
Resistance mechanisms of Trichomonas vaginalis to metronidazole are still not well understood. It has been shown that Mycoplasma hominis has the ability to establish an endosymbiotic relationship with T. vaginalis. This study investigated the association between T. vaginalis and M. hominis symbiosis in relation to metronidazole resistance. This study included 362 pregnant women from the King Edward VIII hospital in South Africa. The women provided self-collected vaginal swabs for the diagnosis of T. vaginalis by culture. Metronidazole susceptibility using the broth-microdilution assay was performed. Detection of the 16S rRNA from M. hominis using T. vaginalis genomic DNA as the template was performed. All statistical analysis was conducted in R statistical computing software. A total of 21 culture positive isolates were obtained resulting in a prevalence of 5.8% for T. vaginalis in the study population. Under anaerobic incubation, 52.4% (11/21) of the isolates were susceptible to metronidazole (MIC ≤ 1 μg/ml). Intermediate resistance (MIC of 2 μg/ml) and full resistance (4 μg/ml) was observed in 38.1% (8/21) and 9.5% (2/21) of the isolates, respectively. The majority of the isolates 95% (19/20) were susceptible to metronidazole under aerobic conditions. Only one isolate had a MIC of 50 μg/ml. M. hominis was shown to be present in 85.7% (18/21) of the T. vaginalis isolates. However, there was no significant association between metronidazole susceptibility and T. vaginalis-M. hominis symbiosis. This study provides evidence of emerging metronidazole resistance in T. vaginalis. However, these resistance profiles were not associated with M. hominis symbiosis.
阴道毛滴虫对甲硝唑的耐药机制尚不清楚。已经表明，支原体具有与阴道毛滴虫建立共生关系的能力。本研究调查了阴道毛滴虫与支原体共生与甲硝唑耐药的关系。本研究纳入了南非爱德华八世国王医院的 362 名孕妇。这些女性提供了自己采集的阴道拭子，用于培养诊断阴道毛滴虫。采用肉汤微量稀释法检测甲硝唑敏感性。使用阴道毛滴虫基因组 DNA 作为模板，检测支原体的 16S rRNA。所有统计分析均在 R 统计计算软件中进行。共获得 21 株培养阳性分离株，研究人群中阴道毛滴虫的患病率为 5.8%。在厌氧孵育条件下，52.4%（11/21）的分离株对甲硝唑敏感（MIC≤1μg/ml）。38.1%（8/21）和 9.5%（2/21）的分离株分别观察到中介耐药（MIC 为 2μg/ml）和完全耐药（4μg/ml）。在有氧条件下，大多数分离株（95%，19/20）对甲硝唑敏感。只有 1 株分离株的 MIC 为 50μg/ml。表明支原体存在于 85.7%（18/21）的阴道毛滴虫分离株中。然而，甲硝唑敏感性与阴道毛滴虫-支原体共生之间没有显著关联。本研究提供了阴道毛滴虫甲硝唑耐药性的出现证据。然而，这些耐药谱与支原体共生无关。
