[辽宁省丹东地区间日疟原虫裂殖子表面蛋白1（PvMSP-1）等位基因分型分析]

[Allele genotype analysis of Plasmodium vivax merozoite surface protein 1 (PvMSP-1) from Dangdong, Liaoning Province].

作者信息

Geng Ying-Zhi, Mao Ling-Ling, Teng Cong, An Chun-Li, Wang Bo, Chen Jing-Yi, Yao Wen-Qing

机构信息

Liaoning Center for Disease Control and Prevention, Shenyang 110005, China.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Jun 30;30(3):242-4.

PMID:23072145

Abstract

Nested PCR method was used to amplify the Plasmodium vivax merozoite surface protein 1 (PvMSP-1) gene fragment containing the ICB5 and ICB6 region from Plasmodium vivax in Liaoning Province. The PCR products were digested by Pvu II restriction endonuclease and the digested fragments were observed by 1.5% agarose gel electrophoresis, and all followed by sequencing analysis and comparison. In 11 field isolates of P. vivax, two kinds of DNA fragments with 470 and 400 bp were produced respectively. After PvuII digestion, two Sal-1 type fragments (120 and 350 bp) were obtained from 5 samples of 470 bp. Single band of 400 bp appeared in 1 samples as Belem type. Two bands of 120 and 280 bp appeared from another 1 sample as recombination type III, and other 4 bands with 120 and 240 bp as Korean isolate. The principal types of PvMSP-1 alleles exist in malaria endemic areas in Liaoning Province with no mixed infection of two different type alleles.

摘要

采用巢式PCR方法从辽宁省间日疟原虫中扩增含ICB5和ICB6区域的间日疟原虫裂殖子表面蛋白1（PvMSP-1）基因片段。PCR产物用Pvu II限制性内切酶消化，消化后的片段用1.5%琼脂糖凝胶电泳观察，随后进行测序分析和比较。在11株间日疟原虫野外分离株中，分别产生了470和400 bp的两种DNA片段。经PvuII消化后，从470 bp的5个样本中获得了两种Sal-1型片段（120和350 bp）。在1个样本中出现400 bp的单带，为贝伦型。在另1个样本中出现120和280 bp的两条带，为重组III型，另外4条带为120和240 bp，为韩国分离株。辽宁省疟疾流行区存在PvMSP-1等位基因的主要类型，无两种不同类型等位基因的混合感染。