Huang Bo, Huang Shiguang, Su Xin-zhuan, Guo Hong, Xu Yucheng, Xu Fei, Hu Xuchu, Yang Yaming, Wang Shanqing, Lu Fangli
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong, China.
Malar J. 2014 Jan 8;13:13. doi: 10.1186/1475-2875-13-13.
Although the numbers of malaria cases in China have been declining in recent years, outbreaks of Plasmodium vivax malaria were still being reported in rural areas south of the Yellow River. To better understand the transmission dynamics of P. vivax parasites in China, the extent of genetic diversity of P. vivax populations circulating in Bozhou of Anhui province of China were investigated using three polymorphic genetic markers: merozoite surface proteins 1 and 3α (pvmsp-1 and pvmsp-3α) and circumsporozoite protein (pvcsp).
Forty-five P. vivax clinical isolates from Bouzhou of Anhui province were collected from 2009 to 2010 and were analysed using PCR/RFLP or DNA sequencing.
Seven and six distinct allelic variants were identified using PCR/RFLP analysis of pvmsp-3α with HhaI and AluI, respectively. DNA sequence analysis of pvmsp-1 (variable block 5) revealed that there were Sal-I and recombinant types but not Belem type, and seven distinct allelic variants in pvmsp-1 were detected, with recombinant subtype 2 (R2) being predominant (66.7%). All the isolates carried pvcsp with VK210 type but not VK247 or P. vivax-like types in the samples. Sequence analysis of pvcsp gene revealed 12 distinct allelic variants, with VK210-1 being predominant (41.5%).
The present data indicate that there is some degree of genetic diversity among P. vivax populations in Anhui province of China. The genetic data obtained may assist in the surveillance of P. vivax infection in endemic areas or in tracking potential future disease outbreak.
尽管近年来中国疟疾病例数呈下降趋势，但黄河以南农村地区仍有间日疟原虫疟疾暴发的报道。为了更好地了解中国间日疟原虫寄生虫的传播动态，利用三种多态性遗传标记：裂殖子表面蛋白1和3α（pvmsp-1和pvmsp-3α）以及环子孢子蛋白（pvcsp），对中国安徽省亳州市流行的间日疟原虫种群的遗传多样性程度进行了调查。
收集了2009年至2010年来自安徽省亳州市的45株间日疟原虫临床分离株，并采用PCR/RFLP或DNA测序进行分析。
分别用HhaI和AluI对pvmsp-3α进行PCR/RFLP分析，鉴定出7个和6个不同的等位基因变体。pvmsp-1（可变区5）的DNA序列分析显示存在Sal-I型和重组型，但不存在贝伦型，在pvmsp-1中检测到7个不同的等位基因变体，其中重组亚型2（R2）占主导（66.7%）。所有分离株均携带VK210型的pvcsp，样本中未检测到VK247型或间日疟原虫样类型。pvcsp基因的序列分析显示有12个不同的等位基因变体，其中VK210-1占主导（41.5%）。
目前的数据表明，中国安徽省间日疟原虫种群存在一定程度的遗传多样性。获得的遗传数据可能有助于对流行地区间日疟原虫感染进行监测或追踪未来潜在的疾病暴发。
