Gao Yun, Yang Xiao-Dong, Wang Li-Na, Li Li-Na, Chen Xiao-Ningl, Zhao Xiang-Ju, Liu Dan
Department of Parasitology, Chengde Medical College, Chengde 067000, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Aug 30;30(4):290-3.
To evaluate the efficacy of tribendimidine (TBD) against 3 geographical isolates of Trichinella spiralis in mice.
Isolates of T. spiralis from Henan (hereinafter referred to as HnT.s), Yunnan (referred to as YnT.s) and Heilongjiang (referred to as HIjT.s) were used in the study. 144 Kunming strain mice were divided into 2 groups: 72 mice in group A (adult stage, treatment at 5 d after infection), and 72 mice in group B (encapsulated larva stage, treatment at 53 d after infection). Group A was further divided equally into 12 sub-groups. Mice in every 3 sub-groups were each infected orally with 200 T. spiralis larvae of the 3 isolates respectively, and the remained 3 subgroups served as untreated control. Mice in the 3 sub-groups infected with one isolate were orally treated with TBD at a single dose of 10, 20, and 30 mg/kg, respectively. Group B was treated as group A but with a course of TBD once daily at a dose of 100, 200, and 300 mg/(kg x d) for 7 d, respectively. Mice in group A were sacrificed 2 d post-treatment and adult worms were recovered from the small intestine and counted. Those in group B were sacrificed 10 d after completion of 7 d treatment. The intact diaphragm was removed and digested for collecting larvae. Worm burden and worm reduction of each treated sub-group were calculated and statistically compared with the respective control.
In group A, the mean worm burden in the treated sub-groups infected with HnT.s and YnT.s were all significantly lower than that of the controls (P < 0.01), with a mean worm reduction rate of 39.0%, 57.9%, and 86.0% in HnT.s sub-groups, and of 34.9%, 69.3%, and 92.2% in YnT.s sub-groups, respectively, showing an increase with the dosage, 2 mice in each of the 30mg/kg sub-groups were cured. The worm burden in the 10 mg/kg of HljT.s subgroup was similar to that of the control (P > 0.05), but was significantly lower in the other 2 sub-groups than that of the controls (P < 0.01). The worm reduction rate in the 3 sub-groups was 27.9%, 57.4%, and 60.7%, respectively. In all treated sub-groups of group B, the mean worm burden was significantly lower than that of the controls (P < 0.05), with a mean worm reduction rate of 57.8%, 75.4%, and 87.5% in HnT.s sub-groups, of 74.5%, 92.4%, and 99.1% in YnT.s sub-groups, and of 50.5%, 53.3%, and 61.6% in HljT.s sub-groups, respectively, with the 3 dosages.
Tribendimidine shows adequate efficacy on Trichinella spiralis adults and on encapsulated larvae of the 3 geographical isolates in mice, with better effect on Yunnan isolate.
评价三苯双脒（TBD）对小鼠体内3个旋毛虫地理隔离株的疗效。
本研究采用来自河南（以下简称HnT.s）、云南（简称YnT.s）和黑龙江（简称HljT.s）的旋毛虫隔离株。144只昆明种小鼠分为2组：A组72只（成虫期，感染后5 d治疗），B组72只（包囊幼虫期，感染后53 d治疗）。A组再平均分为12个亚组。每3个亚组的小鼠分别经口感染3个隔离株的200条旋毛虫幼虫，其余3个亚组作为未治疗对照。感染一个隔离株的3个亚组小鼠分别以10、20和30 mg/kg的单剂量口服TBD进行治疗。B组治疗方法同A组，但TBD疗程为每日1次，剂量分别为100、200和300 mg/（kg·d），共7 d。A组小鼠治疗后2 d处死，从小肠中回收成虫并计数。B组小鼠在7 d治疗结束后10 d处死。取出完整的膈肌进行消化以收集幼虫。计算各治疗亚组的虫荷和减虫率，并与各自的对照组进行统计学比较。
在A组中，感染HnT.s和YnT.s的治疗亚组的平均虫荷均显著低于对照组（P < 0.01），HnT.s亚组的平均减虫率分别为39.0%、57.9%和86.0%，YnT.s亚组分别为34.9%、69.3%和92.2%，均随剂量增加而升高，30 mg/kg亚组各有2只小鼠治愈。HljT.s 10 mg/kg亚组虫荷与对照组相似（P > 0.05），但其他2个亚组显著低于对照组（P < 0.01）。3个亚组的减虫率分别为27.9%、57.4%和60.7%。在B组所有治疗亚组中，平均虫荷均显著低于对照组（P < 0.05），HnT.s亚组的平均减虫率分别为57.8%、75.4%和87.5%，YnT.s亚组分别为74.5%、92.4%和99.1%，HljT.s亚组分别为50.5%、5%和61.6%。
三苯双脒对小鼠体内旋毛虫成虫及3个地理隔离株的包囊幼虫均有良好疗效，对云南隔离株效果更佳。
