Minato M, Honda M, Hatano M
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Nihon Naibunpi Gakkai Zasshi. 1990 Jan 20;66(1):29-41. doi: 10.1507/endocrine1927.66.1_29.
In order to evaluate the hypertensinogenic action of 19-hydroxyandrostenedione (19-OH-AD), which has been reported to be an amplifier of mineralocorticoid, the changes in several humoral factors were observed in 19-OH-AD treated rats as compared to those in DOCA hypertensive rats. Twenty-five male Wistar rats were castrated at 11 weeks of age, and the experiments were begun at 12 weeks of age. The rats were divided into 3 groups. The control group (n = 8) was given an s.c. injection of 0.2 ml of sesame oil. The 19-OH-AD group (n = 10) was injected s.c. with 10 mg of 19-OH-AD dissolved in 0.2 ml of sesame oil, and the DOCA group (n = 8) was injected s.c. with 10 mg of DOCA dissolved in 0.2 ml of sesame oil three time weekly. The urine was collected for a period of 24 hours, and the urine volume, and urinary excretions of electrolytes, prostaglandin E2 (PGE2), kinin and catecholamine were measured before and after the start of the experiment. The systolic blood pressure (S.B.P.) was measured by the tail-cuff method. The S.B.P. values before and at 9 weeks after the start of the experiments were 136.7 +/- 3.8 and 156.0 +/- 2.6 mmHg in the 19-OH-AD group, and 140.6 +/- 5.6 and 179.3 +/- 5.5 mmHg in the DOCA group, respectively. Body weight, which was elevated in both groups, was higher in the 19-OH-AD group than in the DOCA group. Water intake and urine volume were significantly (p less than 0.001) increased only in the DOCA group. The urinary Na/K ratio was significantly (p less than 0.001) elevated in the DOCA group as compared to that in the other two groups. However, there was no significant difference in urinary Na/K ratio between the control and 19-OH-AD groups. The urinary PGE2 and kinin excretions were significantly (p less than 0.01) increased in the DOCA group but did not change appreciably in the 19-OH-AD group. The urinary catecholamine excretion was significantly increased in the DOCA group. However, there were no differences in the catecholamine excretion between the control and 19-OH-AD groups.(ABSTRACT TRUNCATED AT 400 WORDS)
为了评估据报道作为盐皮质激素放大器的19-羟基雄烯二酮（19-OH-AD）的致高血压作用，观察了19-OH-AD处理大鼠与去氧皮质酮（DOCA）高血压大鼠相比几种体液因子的变化。25只雄性Wistar大鼠在11周龄时去势，实验于12周龄开始。大鼠分为3组。对照组（n = 8）皮下注射0.2 ml芝麻油。19-OH-AD组（n = 10）皮下注射溶于0.2 ml芝麻油的10 mg 19-OH-AD，DOCA组（n = 8）每周3次皮下注射溶于0.2 ml芝麻油的10 mg DOCA。收集24小时尿液，测量实验开始前后的尿量以及电解质、前列腺素E2（PGE2）、激肽和儿茶酚胺的尿排泄量。用尾袖法测量收缩压（S.B.P.）。实验开始前和开始后9周时，19-OH-AD组的S.B.P.值分别为136.7±3.8和156.0±2.6 mmHg，DOCA组分别为140.6±5.6和179.3±5.5 mmHg。两组体重均升高，19-OH-AD组高于DOCA组。仅DOCA组的水摄入量和尿量显著增加（p＜0.001）。与其他两组相比，DOCA组的尿钠/钾比值显著升高（p＜0.001）。然而，对照组和19-OH-AD组之间的尿钠/钾比值无显著差异。DOCA组的尿PGE2和激肽排泄量显著增加（p＜0.01），而19-OH-AD组无明显变化。DOCA组的尿儿茶酚胺排泄量显著增加。然而，对照组和19-OH-AD组之间的儿茶酚胺排泄量无差异。（摘要截断于400字）
