Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Department Biología Celular, Genética y Fisiología, Universidad de Málaga, Málaga, Spain.
Plant Signal Behav. 2012 Dec;7(12):1705-8. doi: 10.4161/psb.22100. Epub 2012 Oct 16.
Geminiviruses are ssDNA plant viruses that infect a wide range of crops. Since geminiviruses often infect terminally differentiated cells, they must induce cell cycle re-entry in order to replicate; until recently, only two viral proteins, the replication-associated protein Rep and the curtoviral pathogenicity factor C4, had been assigned a role in the restoration of cell competency. In a recent work, we demonstrated that C2 from Beet curly top virus activates the expression of host genes involved in DNA replication and/or control of the G2/M transition in a manner consistent with cell cycle re-entry. As expected, expression of BCTV C2 results in enhanced replication of DNA viruses. We conclude that BCTV C2 acts as a re-activator of the cell cycle in infected cells, enhancing the DNA replication competency and providing a cell environment favorable for replication of geminiviruses. Potential mechanisms for this novel function are discussed in light of our findings.
双生病毒是感染广泛作物的 ssDNA 植物病毒。由于双生病毒通常感染终末分化细胞，它们必须诱导细胞周期重新进入，以便进行复制；直到最近，只有两种病毒蛋白，复制相关蛋白 Rep 和卷曲病毒致病性因子 C4，被认为在恢复细胞能力方面发挥作用。在最近的一项工作中，我们证明了甜菜曲顶病毒的 C2 通过一种与细胞周期重新进入一致的方式激活涉及 DNA 复制和/或控制 G2/M 转换的宿主基因的表达。正如预期的那样，BCTV C2 的表达导致 DNA 病毒的复制增强。我们得出结论，BCTV C2 作为感染细胞中细胞周期的再激活剂，增强了 DNA 复制能力，并为双生病毒的复制提供了有利的细胞环境。根据我们的发现，讨论了这种新功能的潜在机制。
