Laboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, PR China.
Bioresour Technol. 2012 Dec;126:80-6. doi: 10.1016/j.biortech.2012.09.055. Epub 2012 Sep 25.
The characterization of a spore laccase from Bacillus vallismortis fmb-103, isolated from textile industry disposal sites, is described. The activity was 6.5 U/g of dry spore with ABTS as the substrate. The enzyme was quite stable at high temperature. It retained more than 90% of its initial activity after 10h at 70 °C. The enzyme demonstrated broad pH stability in both acidic and alkaline conditions. There was almost no activity loss at pH 3 over an extended period of time, and the relative activity remained at 82% and 38% at pH 7 and pH 9 after 10 days. NaN(3), SDS, L-cysterine, Dithiothreitol, EDTA and NaCl inhibit the enzyme activity. Triphenylmethane dyes, including malachite green, brilliant green and aniline blue were efficiently degraded by the enzyme after 24h in combination with a mediator with efficiencies of 76.84%, 96.56% and 81.17%, respectively. The reusability of spore laccase for decolorization dyes was also examined.
从纺织工业处理场地分离得到的芽孢杆菌（Bacillus vallismortis fmb-103）的孢子漆酶的特性描述如下。以 ABTS 为底物时，其比活为 6.5 U/g 干孢子。该酶在高温下相当稳定，在 70°C 下孵育 10h 后，仍保留超过 90%的初始活性。该酶在酸性和碱性条件下均具有较宽的 pH 稳定性，在 pH 3 下长时间几乎没有活性损失，10 天后在 pH 7 和 pH 9 下相对活性分别保持在 82%和 38%。NaN(3)、SDS、L-胱氨酸、Dithiothreitol、EDTA 和 NaCl 抑制酶活性。漆酶与介体结合 24h 后，可有效降解包括孔雀石绿、亮绿和苯胺蓝在内的三苯甲烷染料，效率分别为 76.84%、96.56%和 81.17%。还考察了孢子漆酶用于染料脱色的可重复使用性。
