Industrial Biotechnology and Environmental Engineering Group "BiotecnIA", Chemical Engineering Department, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain.
Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, Bl 16, São Paulo, 05508-900, Brazil.
Curr Microbiol. 2020 May;77(5):702-709. doi: 10.1007/s00284-019-01856-9. Epub 2020 Jan 2.
In this work we investigated the enzymes produced by Bacillus aryabhattai BA03, particularly in those involved in ligninolytic activities such as laccases (Lac), lignin peroxidases (LiP) and Mn-dependent peroxidase (MnD-P). In this way, the maximal production of Lac (0.069 ± 0.000 U/mL) was obtained at pH 9, and 37 ºC after 72 h. LiP expressed the highest activity at 96 h in acid medium at 37 ºC (0.741 ± 0.029 U/mL). Meanwhile, the strain produced MnD-P (1.052 ± 0.001 U/mL) at the highest temperature assayed (44 ºC) and pH 7 at 72 h. In addition, this microorganism produced resistant endospores able to germinate after the sterilization program (121 ºC, 15 min) showing a high enzymatic activity. Using the heat-treated culture as inoculum, the percentage of decolorization of 150 mg/L of Coomassie Brillant Blue reached 89.42 ± 0.11% in only 24 h. These results open the use of these enzymes and endospores in bioremediation processes carried out under different temperatures and pH values.
在这项工作中，我们研究了芽孢杆菌 BA03 产生的酶，特别是那些参与木质素降解活性的酶，如漆酶（Lac）、木质素过氧化物酶（LiP）和锰依赖过氧化物酶（MnD-P）。这样，在 pH 9 和 37°C 下 72 小时后，Lac（0.069±0.000 U/mL）的最大产量得以实现。LiP 在 37°C 的酸性介质中在 96 小时时表达出最高的活性（0.741±0.029 U/mL）。同时，该菌株在最高温度（44°C）和 pH 7 下在 72 小时时产生 MnD-P（1.052±0.001 U/mL）。此外，这种微生物产生了耐热芽孢，能够在灭菌程序（121°C，15 分钟）后发芽，表现出高酶活性。使用热处理后的培养物作为接种物，在 24 小时内，150mg/L 的考马斯亮蓝的脱色率达到了 89.42±0.11%。这些结果为在不同温度和 pH 值下进行的生物修复过程中使用这些酶和芽孢开辟了道路。
