Shan Guang, Tang Tian, Zhang Duanlian
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):713-718. doi: 10.1007/s11596-012-1023-5. Epub 2012 Oct 18.
This study examined the roles of HLA-G in the pathogenesis, development and immune tolerance of hemangioma. From 2000 to 2007, 52 paraffin-embedded specimens (26 from males and 26 from females) of skin capillary hemangioma and 7 samples of adjacent normal skin tissues were collected. Four fresh specimens of hemangioma were also harvested. All samples were HE-stained and proliferative cell nuclear antigen (PCNA) was immunohistochemically detected by using SP method. The samples were classified into proliferative group and degenerative group according to the Mulliken criteria and the expression pattern of PCNA. SP method and quantum dots double staining were applied to detect the expression of HLA-G and PCNA in hemangioma and normal tissue samples. The expression of HLA-G was detected by RT-PCR. The results showed that among the 52 samples of hemangioma, 29 were of proliferative type and 23 degenerative type, and of the four fresh samples of hemangioma, 2 were of proliferative type and 2 degenerative type. SP method results showed that HLA-G was expressed in both proliferative and degenerative hemangioma, but not in normal tissues. The quantum dots double staining exhibited that HLA-G expression was significantly higher in proliferative group than in degenerative (P<0.05) and normal groups (P<0.05), but there was no statistically significant difference between the latter two groups (P>0.05). RT-PCR revealed that HLA-G was transcribed in both the proliferative and degenerative hemangioma tissues, but not in normal tissues. We are led to conclude that the elevated expression of HLA-G in proliferative hemangioma cells may lead to immune tolerance, which allows cells to escape immune surveillance and proliferate. On the other hand, the lower expression of HLA-G in degenerative hemangioma may result in immune cells-induced degeneration of hemangioma.
本研究探讨了HLA - G在血管瘤发病机制、发展过程及免疫耐受中的作用。2000年至2007年，收集了52例皮肤毛细血管瘤石蜡包埋标本（男性26例，女性26例）及7例相邻正常皮肤组织样本。还采集了4例新鲜血管瘤标本。所有样本均进行苏木精 - 伊红（HE）染色，并采用链霉菌抗生物素蛋白 - 过氧化物酶（SP）法免疫组化检测增殖细胞核抗原（PCNA）。根据Mulliken标准和PCNA表达模式将样本分为增殖组和退化组。应用SP法和量子点双重染色检测血管瘤和正常组织样本中HLA - G和PCNA的表达。通过逆转录 - 聚合酶链反应（RT - PCR）检测HLA - G的表达。结果显示，在52例血管瘤样本中，29例为增殖型，23例为退化型，4例新鲜血管瘤样本中，2例为增殖型，2例为退化型。SP法结果显示，HLA - G在增殖型和退化型血管瘤中均有表达，但在正常组织中无表达。量子点双重染色显示，增殖组中HLA - G表达明显高于退化组（P < 0.05）和正常组（P < 0.05），但后两组之间无统计学差异（P > 0.05）。RT - PCR显示，HLA - G在增殖型和退化型血管瘤组织中均有转录，但在正常组织中无转录。我们得出结论，增殖型血管瘤细胞中HLA - G表达升高可能导致免疫耐受，使细胞逃避免疫监视并增殖。另一方面，退化型血管瘤中HLA - G表达较低可能导致免疫细胞诱导的血管瘤退化。
