Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, 18618-970 São Paulo, Brazil.
Phytother Res. 2013 Sep;27(9):1277-81. doi: 10.1002/ptr.4861. Epub 2012 Oct 17.
Osteosarcoma (OSA) is a primary bone neoplasm frequently diagnosed in dogs. The biology of OSA in pet dogs is identical to that of pediatric patients, and it has been considered an excellent model in vivo to study human OSA. Since the individual response to chemotherapy is unpredictable and considering that propolis is a natural product with several biological properties, this work evaluated the cytotoxic action of propolis on canine OSA cells. The primary cell culture of canine OSA was obtained from the tumor of a dog with OSA. Cell viability was assessed after incubation with propolis, 70% ethanol (propolis solvent), and carboplatin after 6, 24, 48, and 72 h. Cell viability was analyzed by the crystal violet method. Data showed that canine OSA cells were sensitive to propolis in a dose- and time-dependent manner and had a distinct morphology compared to control. Its solvent (70% ethanol) had no effect on cell viability, suggesting that the cytotoxic action was exclusively due to propolis. Our propolis sample exerted a cytotoxic effect on canine OSA cells, and its introduction as a possible therapeutic agent in vivo could be investigated, providing a new contribution to OSA treatment.
骨肉瘤（OSA）是一种常见于犬类的原发性骨肿瘤。宠物狗的 OSA 生物学与儿科患者相同，因此被认为是研究人类 OSA 的理想体内模型。由于个体对化疗的反应不可预测，并且考虑到蜂胶是一种具有多种生物学特性的天然产物，因此这项工作评估了蜂胶对犬骨肉瘤细胞的细胞毒性作用。从患有 OSA 的犬的肿瘤中获得犬骨肉瘤的原代细胞培养物。在用蜂胶、70%乙醇（蜂胶溶剂）和卡铂孵育 6、24、48 和 72 小时后，评估细胞活力。通过结晶紫法分析细胞活力。数据显示，犬骨肉瘤细胞对蜂胶具有剂量和时间依赖性的敏感性，与对照组相比具有明显的形态。其溶剂（70%乙醇）对细胞活力没有影响，表明细胞毒性作用仅归因于蜂胶。我们的蜂胶样品对犬骨肉瘤细胞具有细胞毒性作用，因此可以研究其作为体内可能的治疗剂的引入，为 OSA 的治疗提供新的贡献。
