Faculty of Veterinary Medicine, Antonio Nariño University, Bogotá, Colombia.
Institute of Biosciences, São Paulo State University (UNESP), Campus Botucatu, Brazil.
Phytother Res. 2019 Mar;33(3):591-601. doi: 10.1002/ptr.6246. Epub 2018 Nov 28.
Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC-MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC ). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH-DA. Transwell assay was used to evaluate the invasiveness of propolis-treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.
骨肉瘤（OSA）是一种具有侵袭性生物学行为和转移性进展的骨癌。由于蜂胶具有开发新型抗肿瘤药物的潜力，我们评估了哥伦比亚蜂胶样品的化学成分及其对 OSA 细胞细胞毒性作用的机制。采用 GC-MS 分析化学成分，用 DPPH 自由基清除活性进行测量。采用聚类和主成分分析建立与抑制浓度 50%（IC ）的相关性。通过 MTT 测定分析细胞活力；通过流式细胞术测定细胞凋亡；通过 rhodamine 123 和 DCFH-DA 评估线粒体膜通透性和活性氧。采用 Transwell 测定评估蜂胶处理细胞的侵袭性。样品分组：簇 1 含有二萜和二苯甲酮，具有最高的抗自由基活性；簇 2 的特征是三萜、脂肪酸和二萜。Usm 含有与其他样品不同的二萜和三萜，Sil 含有三萜和类黄酮。细胞凋亡、线粒体膜改变和抑制细胞侵袭是体外抑制 OSA 细胞的主要机制，表明哥伦比亚蜂胶具有发现新型抗肿瘤药物的潜力。
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