分段标记法研究多结构域蛋白。

Segmental labeling to study multidomain proteins.

机构信息

Department of Chemistry, State University of New York, Albany, NY 12222, USA.

出版信息

Adv Exp Med Biol. 2012;992:17-33. doi: 10.1007/978-94-007-4954-2_2.

Abstract

This chapter contains a review of methodologies and recent applications of segmental labeling for NMR structural studies of proteins and protein complexes. Segmental labeling is used to specifically label a segment of protein structure with NMR active nuclei, thus reducing NMR spectral complexity and greatly facilitating structural NMR studies of large multi-domain proteins. It can also be used to introduce a synthetic fragment into a protein structure to study post-translationally modified proteins. Detailed protocols describing segmental labeling techniques are also included.

摘要

本章综述了蛋白质和蛋白质复合物结构的 NMR 研究中分段标记的方法和最新应用。分段标记用于用 NMR 活性核特异性标记蛋白质结构的片段，从而降低 NMR 谱复杂性，并极大地促进了大的多结构域蛋白质的结构 NMR 研究。它还可用于在蛋白质结构中引入合成片段来研究翻译后修饰的蛋白质。本章还包括了详细的分段标记技术描述。