实验性猪圆环病毒 2 型（PCV2）攻毒后，经改良的灭活嵌合 PCV1-2 疫苗诱导的体液和细胞免疫降低了猪圆环病毒 2 型（PCV2）血症。
Reduction of porcine circovirus type 2 (PCV2) viremia by a reformulated inactivated chimeric PCV1-2 vaccine-induced humoral and cellular immunity after experimental PCV2 challenge.
机构信息
College of Veterinary Medicine, Department of Veterinary Pathology, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea.
出版信息
BMC Vet Res. 2012 Oct 19;8:194. doi: 10.1186/1746-6148-8-194.
BACKGROUND
The objective of the present study was to elucidate the humoral and cellular immune response mechanisms by which a reformulated inactivated chimeric PCV1-2 vaccine reduces the PCV2 viremia. Forty PCV2 seronegative 3-week-old pigs were randomly divided into the following four groups: vaccinated challenged (T01), vaccinated non-challenged (T02), non-vaccinated challenged (T03), and non-vaccinated non-challenged (T04) animals. The pigs in groups T01 and T02 were immunized with a reformulated inactivated chimeric PCV1-2 vaccine (Fostera™ PCV; Pfizer Animal Health) administered as a 2.0 ml dose at 21 days of age. At 35 days of age (0 days post-challenge), the pigs in groups T01 and T03 were inoculated intranasally with 2 ml each of PCV2b.
RESULTS
A reduction of PCV2 viremia coincided with the appearance of both PCV2-specific neutralizing antibodies (NA) and interferon-γ-secreting cells (IFN-γ-SCs) in the vaccinated animals. However, the presence of anti-PCV2 IgG antibodies did not correlate with the reduction of PCV2 viremia. Lymphocyte subset analysis indicated that the numbers of CD3+ and CD4+ cells increased in vaccinated animals but the numbers of CD4+ cells decreased transiently in non-vaccinated animals. The observation of a delayed type hypersensitivity response in only the vaccinated animals also supports a CD4+ cell-associated protective cellular immune response induced by the reformulated inactivated chimeric PCV1-2 vaccine.
CONCLUSIONS
The induction of PCV2-specific NA and IFN-γ-SCs, and CD4+ cells by the reformulated inactivated chimeric PCV1-2 vaccine is the important protective immune response leading to reduction of the PCV2 viremia and control of the PCV2 infection. To our knowledge this is the first demonstration of protective humoral and cellular immunity induced by the reformulated inactivated chimeric PCV1-2 vaccine and its effect on reduction of PCV2 viremia by vaccination.
背景
本研究的目的是阐明一种改良的灭活嵌合 PCV1-2 疫苗如何通过体液和细胞免疫应答机制降低 PCV2 病毒血症。将 40 头 PCV2 血清阴性 3 周龄猪随机分为以下四组：接种挑战组（T01）、接种非挑战组（T02）、未接种挑战组（T03）和未接种非挑战组（T04）。T01 和 T02 组猪用改良的灭活嵌合 PCV1-2 疫苗（Fostera™ PCV；辉瑞动物保健）免疫，21 日龄时每头猪肌肉注射 2.0ml 剂量。在 35 日龄（攻毒后 0 天），T01 和 T03 组猪经鼻腔接种各 2ml PCV2b。
结果
接种动物中出现 PCV2 特异性中和抗体（NA）和干扰素-γ 分泌细胞（IFN-γ-SC）与 PCV2 病毒血症减少同时发生。然而，抗 PCV2 IgG 抗体的存在与 PCV2 病毒血症的减少无关。淋巴细胞亚群分析表明，接种动物的 CD3+和 CD4+细胞数量增加，但未接种动物的 CD4+细胞数量短暂减少。仅在接种动物中观察到迟发型超敏反应也支持改良的灭活嵌合 PCV1-2 疫苗诱导的与 CD4+细胞相关的保护性细胞免疫应答。
结论
改良的灭活嵌合 PCV1-2 疫苗诱导的 PCV2 特异性 NA 和 IFN-γ-SC 以及 CD4+细胞是导致 PCV2 病毒血症减少和控制 PCV2 感染的重要保护性免疫应答。据我们所知，这是首次证明改良的灭活嵌合 PCV1-2 疫苗诱导的保护性体液和细胞免疫及其对疫苗接种降低 PCV2 病毒血症的影响。
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