Kim N H, Lim H Y, Im K S, Kim J H, Sur J-H
Department of Veterinary Pathology, Small Animal Tumour Diagnostic Center, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
J Comp Pathol. 2013 May;148(4):298-306. doi: 10.1016/j.jcpa.2012.08.009. Epub 2012 Oct 15.
Molecular-based classification of canine mammary carcinomas (CMCs) has been a recent research focus. In human breast cancer, triple-negative and basal-like phenotypes are distinct molecular subgroups that are known for their poor prognosis, but these tumours are not yet well defined in the dog. The aim of this study was to determine whether CMCs include triple-negative and basal-like phenotypes by immunohistochemical assessment of expression of the oestrogen receptor (OR), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and four basal markers, cytokeratin (CK) 14, CK5/6, p63 and the epidermal growth factor receptor (EGFR). In this study of 241 CMCs, 45 triple-negative tumours (OR(-), PR(-) and HER2(-)) were identified and this phenotype was associated with an unfavourable prognosis. In these tumours, the expression of CK14, CK5/6 and EGFR was related to clinicopathological parameters, while the expression of p63 was not relevant. The majority of the triple-negative tumours were of the basal-like phenotype, given that 75.6% of them expressed more than two basal markers. However, three of the basal markers were not uniformly expressed; therefore, the proportion of the basal-like phenotype was altered on the basis of the selection of the markers. Although both triple-negative and basal-like phenotypes are distinct entities in CMC, further study is needed to differentiate one from the other.
犬乳腺肿瘤（CMCs）基于分子的分类是近期的研究重点。在人类乳腺癌中，三阴性和基底样表型是不同的分子亚组，以预后不良而闻名，但这些肿瘤在犬类中尚未得到很好的定义。本研究的目的是通过免疫组织化学评估雌激素受体（OR）、孕激素受体（PR）、人表皮生长因子受体2（HER2）以及四种基底标志物细胞角蛋白（CK）14、CK5/6、p63和表皮生长因子受体（EGFR）的表达，来确定CMCs是否包括三阴性和基底样表型。在这项对241例CMCs的研究中，鉴定出45例三阴性肿瘤（OR(-)、PR(-)和HER2(-)），这种表型与不良预后相关。在这些肿瘤中，CK14、CK5/6和EGFR的表达与临床病理参数有关，而p63的表达则无关。大多数三阴性肿瘤为基底样表型，因为其中75.6%表达两种以上基底标志物。然而，三种基底标志物并非均匀表达；因此，基底样表型的比例会因标志物的选择而改变。虽然三阴性和基底样表型在CMCs中都是不同的实体，但仍需要进一步研究以区分两者。
