College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Shandong Normal University, Jinan 250014, People's Republic of China.
Biosens Bioelectron. 2013 Mar 15;41:903-6. doi: 10.1016/j.bios.2012.09.048. Epub 2012 Oct 2.
A novel, simple, and versatile amperometric sensing platform was developed based on HRP-mimicking DNAzyme-catalyzed template-guided deposition of polyaniline (PANI). Pb(2+) was chosen as a model to demonstrate the proof-of-concept of our approach. The Pb(2+) aptamer was first self-assembled on the electrode surface. Upon addition of Pb(2+), Pb(2+) bound to its aptamer to form G-quadruplex (G4). HRP-mimicking DNAzyme with peroxidase catalytic activity was successfully generated after hemin was efficiently intercalated into the G4 structure. Subsequently, HRP-mimicking DNAzyme catalyzed oxidation of aniline to PANI with H(2)O(2) exclusively at the G4 structures, which lead to a readily measurable "turn-on" electrochemical signal. The constructed platform exhibited a good linear response toward Pb(2+) over a wide range of concentration from 1.0×10(-9) M to 1.0×10(-6) M with the detection limit of 5.0×10(-10) M. The high performance DNAzyme-based strategy can be further extended to quantify a wide variety of analytes.
基于 HRP 模拟 DNA zyme 催化的模板引导聚苯胺（PANI）沉积，开发了一种新颖、简单、通用的安培传感平台。选择 Pb(2+)作为模型来证明我们方法的概念验证。首先将 Pb(2+)适体自组装到电极表面。加入 Pb(2+)后，Pb(2+)与适体结合形成 G-四链体 (G4)。血红素被有效插入 G4 结构后，成功生成具有过氧化物酶催化活性的 HRP 模拟 DNA 酶。随后，HRP 模拟 DNA 酶在 G4 结构处仅用 H(2)O(2)催化苯胺氧化为 PANI，产生易于测量的“开启”电化学信号。所构建的平台对 Pb(2+)表现出良好的线性响应，浓度范围从 1.0×10(-9) M 到 1.0×10(-6) M，检测限为 5.0×10(-10) M。基于 DNA 酶的高性能策略可以进一步扩展到定量分析各种分析物。
