钙离子通道阻滞剂对豚鼠回肠肌间神经丛中各种类型刺激诱导的乙酰胆碱释放的影响。

Effects of Ca2+ blockers on the various types of stimuli-induced acetylcholine release from guinea pig ileum myenteric plexus.

作者信息

Yokoyama K, Yagasaki O

机构信息

Department of Veterinary Pharmacology, College of Agriculture, University of Osaka Prefecture, Japan.

出版信息

Jpn J Pharmacol. 1990 Jan;52(1):109-14. doi: 10.1254/jjp.52.109.

DOI:10.1254/jjp.52.109

Abstract

In the present study, we investigated the effects of various Ca2+ blockers on the release of acetylcholine (ACh) induced by nicotine, electrical field stimulation (EFS) and high-K+. Cd2+ markedly depressed the ACh release due to these stimuli. Verapamil inhibited the nicotine-induced ACh release remarkably and the EFS- or the high-K(+)-induced ACh release to a lesser extent. Since the nicotine- and the EFS-induced ACh releases were inhibited by procaine, the local anesthetic property of verapamil likely contributes in part to the inhibition. Diltiazem abolished the nicotine-induced ACh release completely but did not affect the EFS-induced release and significantly increased the high-K(+)-induced ACh release. These results suggest the absolute requirement for extracellular Ca2+ in the release of ACh induced by nicotine as well as EFS and high-K+. In addition, these stimuli may open the same Ca2+ channel to evoke ACh release.

摘要

在本研究中，我们研究了各种钙离子阻滞剂对尼古丁、电场刺激（EFS）和高钾诱导的乙酰胆碱（ACh）释放的影响。镉离子（Cd2+）显著抑制了由这些刺激引起的ACh释放。维拉帕米显著抑制尼古丁诱导的ACh释放，对EFS或高钾诱导的ACh释放的抑制作用较小。由于普鲁卡因抑制了尼古丁和EFS诱导的ACh释放，维拉帕米的局部麻醉特性可能部分促成了这种抑制作用。地尔硫卓完全消除了尼古丁诱导的ACh释放，但不影响EFS诱导的释放，且显著增加了高钾诱导的ACh释放。这些结果表明，尼古丁、EFS和高钾诱导的ACh释放绝对需要细胞外钙离子。此外，这些刺激可能打开相同的钙离子通道以引发ACh释放。